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The epitope space o...
The epitope space of the human proteome
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- Berglund, Lisa (författare)
- KTH,Skolan för bioteknologi (BIO)
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- Andrade, Jorge (författare)
- KTH,Skolan för bioteknologi (BIO)
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- Odeberg, Jacob (författare)
- Karolinska Institutet,KTH,Skolan för bioteknologi (BIO)
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- Uhlén, Mathias (författare)
- KTH,Skolan för bioteknologi (BIO)
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(creator_code:org_t)
- Wiley, 2008
- 2008
- Engelska.
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Ingår i: Protein Science. - : Wiley. - 0961-8368 .- 1469-896X. ; 17:4, s. 606-613
- Relaterad länk:
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https://onlinelibrar...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- In the post-genome era, there is a great need for protein-specific affinity reagents to explore the human proteome. Antibodies are suitable as reagents, but generation of antibodies with low cross-reactivity to other human proteins requires careful selection of antigens. Here we show the results from a proteomewide effort to map linear epitopes based on uniqueness relative to the entire human proteome. The analysis was based on a sliding window sequence similarity search using short windows (8, 10, and 12 amino acid residues). A comparison of exact string matching (Hamming distance) and a heuristic method (BLAST) was performed, showing that the heuristic method combined with a grid strategy allows for whole proteome analysis with high accuracy and feasible run times. The analysis shows that it is possible to find unique antigens for a majority of the human proteins, with relatively strict rules involving low sequence identity of the possible linear epitopes. The implications for human antibody-based proteomics efforts are discussed.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
Nyckelord
- proteomics; antigen; epitope; sequence similarity; antibody; grid; B-CELL EPITOPES; ANTIGENIC DETERMINANTS; DATA-BANK; PREDICTION; ANTIBODIES; MYOGLOBIN; PEPTIDES; SEQUENCE; LIBRARIES; PROTEINS
- Bioengineering
- Bioteknik
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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