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Sökning: onr:"swepub:oai:DiVA.org:liu-101813" > Detection of the hy...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003213naa a2200373 4500
001oai:DiVA.org:liu-101813
003SwePub
008131122s2005 | |||||||||||000 ||eng|
024a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-1018132 URI
024a https://doi.org/10.1262/jrd.170102 DOI
040 a (SwePub)liu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
100a Bergqvist, ASu Swedish University of Agriculture Science, Sweden4 aut
2451 0a Detection of the hyaluronan receptor CD44 in the bovine oviductal epithelium
264 1a Ibaraki , Japan :b Society for Reproduction and Development,c 2005
338 a print2 rdacarrier
520 a Hyaluronan is involved in fundamental reproductive events such as sperm storage in the female reproductive tract, fertilization, and early embryo development, these functions are presumably mediated by its major cell surface receptor, CD44. The present study was conducted to investigate the presence and localization of CD44 in the bovine oviductal epithelium, using immunohistochemical and Western blot methods on tissue sections and epithelial cell extracts collected from the uterotubal junction (UTJ), isthmus, and ampulla of animals in the oestrus or luteal phase of the oestrous cycle. While positive immunolabelling for CD44 was found on the ad-luminal surface and supra-nuclear region of epithelial cells in all tubal segments investigated, in the UTJ, there were epithelial cells in which the entire cytoplasm positively stained. We found no differences in terms of CD44-positive staining between the different stages of the oestrous cycle. Presence of CD44 was detected by Western blotting in the tubal epithelium as a single band at 200 kDa. Although it appeared in all tubal segments, the expression of CD44 protein was more accentuated in the sperm reservoir (UTJ) than in the other segments. This is the first time CD44 has been detected in the epithelium of the tubal sperm reservoir in cattle, suggesting a pathway for the action of hyaluronan in this segment.
650 7a LANTBRUKSVETENSKAPERx Bioteknologi med applikationer på växter och djur0 (SwePub)4042 hsv//swe
650 7a AGRICULTURAL SCIENCESx Agricultural Biotechnology0 (SwePub)4042 hsv//eng
653 a CD44; hyaluronan; bovine; oviduct
653 a TECHNOLOGY
653 a TEKNIKVETENSKAP
700a Yokoo, Mu Tohoku University , Japan;4 aut
700a Bage, Ru Swedish University of Agriculture Science, Sweden4 aut
700a Sato, Eu Tohoku University, Japan;4 aut
700a Rodriguez-Martinez, Heribertou Swedish University of Agriculture Science, Sweden4 aut0 (Swepub:liu)herro55
710a Swedish University of Agriculture Science, Swedenb Tohoku University , Japan;4 org
773t Journal of reproduction and developmentd Ibaraki , Japan : Society for Reproduction and Developmentg 51:4, s. 445-453q 51:4<445-453x 0916-8818x 1348-4400
856u https://www.jstage.jst.go.jp/article/jrd/51/4/51_4_445/_pdf
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-101813
8564 8u https://doi.org/10.1262/jrd.17010

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