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A miniaturized nano...
A miniaturized nanobiosensor for choline analysis
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- Pal, Souvik (författare)
- BITS, Goa, India
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- Kumar Sharma, Manoj (författare)
- Indian Institute of Technology Delhi, India
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- Danielsson, Bengt (författare)
- Acromed Invest AB, Lund, Sweden
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- Willander, Magnus (författare)
- Linköpings universitet,Fysik och elektroteknik,Tekniska högskolan
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- Chatterjee, Ratnamala (författare)
- Indian Institute of Technology Delhi, India
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- Bhand, Sunil (författare)
- BITS, Goa, India
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(creator_code:org_t)
- Elsevier, 2014
- 2014
- Engelska.
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Ingår i: Biosensors & bioelectronics. - : Elsevier. - 0956-5663 .- 1873-4235. ; 54, s. 558-564
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- A novel reusable chemiluminescence choline nanobiosensor has been developed using aligned zinc oxide nanorod-films (ZnONR). The chemically fashioned ZnONR were synthesized by hybrid wet chemical route onto glass substrates and used to fabricate a stable chemiluminescent choline biosensor. The biosensor was constructed by co-immobilization of the enzymes choline oxidase and peroxidase. The covalent immobilization of the enzymes on the ZnONR was achieved using 16-phosphonohexadecanoic acid as a cross-linker. The phosphonation of the ZnONR imparted significant stability to the immobilized enzyme as against physisorbed enzyme. A lower value of Michaelis-Menten constant (K-m), of 0.062 mM for the covalently coupled enzyme over the physisorbed enzymes facilitated enhanced stability of ZnONR nanobiosensor. The ZnONR-choline biosensor has been investigated over a wide range of choline from 0.0005 mM to 2 mM. Importantly, the recovery of choline in milk samples was close to 99%. Using the developed biosensor, choline was measurable even after 30 days with 60 repeated measurements proving the stability of the sensor (Intraday RSD%=2.83 and Interday RSD%=3.51).
Nyckelord
- Choline; Nanobiosensor; Zinc oxide nanorod; Chemiluminescence; Phosphonation; Milk
- TECHNOLOGY
- TEKNIKVETENSKAP
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