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Regulatory and Func...
Regulatory and Functional Connection of Microphthalmia-Associated Transcription Factor and Anti-Metastatic Pigment Epithelium Derived Factor in Melanoma
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- Fernandez-Barral, Asuncion (författare)
- University of Autonoma Madrid, Spain CSIC UAM Madrid, Spain
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- Luis Orgaz, Jose (författare)
- University of Autonoma Madrid, Spain CSIC UAM Madrid, Spain Kings Coll London, England
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- Baquero, Pablo (författare)
- University of Autonoma Madrid, Spain CSIC UAM Madrid, Spain University of Glasgow, Scotland
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- Ali, Zaheer (författare)
- Linköpings universitet,Avdelningen för kardiovaskulär medicin,Hälsouniversitetet
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- Moreno, Alberto (författare)
- CSIC UAM Madrid, Spain University of Dundee, Scotland
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- Tiana, Maria (författare)
- University of Autonoma Madrid, Spain CSIC UAM Madrid, Spain
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- Gomez, Valenti (författare)
- University of Autonoma Madrid, Spain CSIC UAM Madrid, Spain UCL, England
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- Riveiro-Falkenbach, Erica (författare)
- University of Complutense Madrid, Spain Institute Invest I 12, Spain
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- Canadas, Carmen (författare)
- Capio Fdn Jimenez Diaz, Spain
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- Zazo, Sandra (författare)
- Capio Fdn Jimenez Diaz, Spain
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- Bertolotto, Corine (författare)
- CHU Nice, France CHU Nice, France
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- Davidson, Irwin (författare)
- University of Strasbourg, France
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- Luis Rodriguez-Peralto, Jose (författare)
- University of Complutense Madrid, Spain Institute Invest I 12, Spain
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- Palmero, Ignacio (författare)
- CSIC UAM Madrid, Spain
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- Rojo, Federico (författare)
- Capio Fdn Jimenez Diaz, Spain
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- Jensen, Lasse (författare)
- Karolinska Institutet,Linköpings universitet,Avdelningen för kardiovaskulär medicin,Hälsouniversitetet
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- del Peso, Luis (författare)
- University of Autonoma Madrid, Spain CSIC UAM Madrid, Spain
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- Jimenez, Benilde (författare)
- University of Autonoma Madrid, Spain CSIC UAM Madrid, Spain Institute Invest I 12, Spain
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(creator_code:org_t)
- Neoplasia, 2014
- 2014
- Engelska.
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Ingår i: Neoplasia. - : Neoplasia. - 1522-8002 .- 1476-5586. ; 16:6, s. 529-542
- Relaterad länk:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Pigment epithelium-derived factor (PEDF), a member of the serine protease inhibitor superfamily, has potent anti-metastatic effects in cutaneous melanoma through its direct actions on endothelial and melanoma cells. Here we show that PEDF expression positively correlates with microphthalmia-associated transcription factor ( MITF) in melanoma cell lines and human samples. High PEDF and MITF expression is characteristic of low aggressive melanomas classified according to molecular and pathological criteria, whereas both factors are decreased in senescent melanocytes and naevi. Importantly, MITF silencing down-regulates PEDF expression in melanoma cell lines and primary melanocytes, suggesting that the correlation in the expression reflects a causal relationship. In agreement, analysis of Chromatin immunoprecipitation coupled to high throughput sequencing (ChIP-seq) data sets revealed three MITF binding regions within the first intron of SERPINF1, and reporter assays demonstrated that the binding of MITF to these regions is sufficient to drive transcription. Finally, we demonstrate that exogenous PEDF expression efficiently halts in vitro migration and invasion, as well as in vivo dissemination of melanoma cells induced by MITF silencing. In summary, these results identify PEDF as a novel transcriptional target of MITF and support a relevant functional role for the MITF-PEDF axis in the biology of melanoma.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Fernandez-Barral ...
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Luis Orgaz, Jose
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Baquero, Pablo
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Ali, Zaheer
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Moreno, Alberto
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Tiana, Maria
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visa fler...
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Gomez, Valenti
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Riveiro-Falkenba ...
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Canadas, Carmen
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Zazo, Sandra
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Bertolotto, Cori ...
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Davidson, Irwin
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Luis Rodriguez-P ...
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Palmero, Ignacio
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Rojo, Federico
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Jensen, Lasse
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del Peso, Luis
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Jimenez, Benilde
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
- Artiklar i publikationen
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Neoplasia
- Av lärosätet
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Linköpings universitet
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Karolinska Institutet