Sökning: onr:"swepub:oai:DiVA.org:liu-120047" > Effect of Storage T...
Fältnamn | Indikatorer | Metadata |
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000 | 04089naa a2200421 4500 | |
001 | oai:DiVA.org:liu-120047 | |
003 | SwePub | |
008 | 150706s2015 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-1200472 URI |
024 | 7 | a https://doi.org/10.1371/journal.pone.01283062 DOI |
040 | a (SwePub)liu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Islam, Rakibulu University of Oslo, Norway; Oslo University Hospital, Norway; Harvard University, MA USA4 aut |
245 | 1 0 | a Effect of Storage Temperature on Structure and Function of Cultured Human Oral Keratinocytes |
264 | c 2015-06-08 | |
264 | 1 | b Public Library of Science,c 2015 |
338 | a electronic2 rdacarrier | |
500 | a Funding Agencies|Department of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo, Norway [5142001] | |
520 | a Purpose/Aims To assess the effect of storage temperature on the viability, phenotype, metabolism, and morphology of cultured human oral keratinocytes (HOK). Materials and Methods Cultured HOK cells were stored in HEPES- and sodium bicarbonate-buffered Minimum Essential Medium (MEM) at nine temperatures in approximately 4 degrees C increments from 4 degrees C to 37 degrees C for seven days. Cells were characterized for viability by calcein fluorescence, phenotype retention by immunocytochemistry, metabolic parameters (pH, glucose, lactate, and O-2) within the storage medium by blood gas analysis, and morphology by scanning electron microscopy and light microscopy. Results Relative to the cultured, but non-stored control cells, a high percentage of viable cells were retained only in the 12 degrees C and 16 degrees C storage groups (85%+/- 13% and 68%+/- 10%, respectively). Expression of ABCG2, Bmi1, C/EBP delta, PCNA, cytokeratin 18, and caspase-3 were preserved after storage in the 5 groups between 4 degrees C and 20 degrees C, compared to the non-stored control. Glucose, pH and pO(2) in the storage medium declined, whereas lactate increased with increasing storage temperature. Morphology was best preserved following storage of the three groups between 12 degrees C, 16 degrees C, and 20 degrees C. Conclusion We conclude that storage temperatures of 12 degrees C and 16 degrees C were optimal for maintenance of cell viability, phenotype, and morphology of cultured HOK. The storage method described in the present study may be applicable for other cell types and tissues; thus its significance may extend beyond HOK and the field of ophthalmology. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicin0 (SwePub)3022 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicine0 (SwePub)3022 hsv//eng |
700 | 1 | a Jackson, Catherineu Oslo University Hospital, Norway4 aut |
700 | 1 | a Eidet, Jon R.u Oslo University Hospital, Norway4 aut |
700 | 1 | a Messelt, Edward B.u University of Oslo, Norway4 aut |
700 | 1 | a Maria Corraya, Rimau Oslo University Hospital, Norway; Harvard University, MA USA4 aut |
700 | 1 | a Lyberg, Torsteinu Oslo University Hospital, Norway4 aut |
700 | 1 | a Griffith, Mayu Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten4 aut0 (Swepub:liu)maygr76 |
700 | 1 | a Dartt, Darlene A.u Harvard University, MA USA4 aut |
700 | 1 | a Utheim, Tor P.u University of Oslo, Norway; Oslo University Hospital, Norway; Harvard University, MA USA4 aut |
710 | 2 | a University of Oslo, Norway; Oslo University Hospital, Norway; Harvard University, MA USAb Oslo University Hospital, Norway4 org |
773 | 0 | t PLOS ONEd : Public Library of Scienceg 10:6, s. e0128306-q 10:6<e0128306-x 1932-6203 |
856 | 4 | u https://liu.diva-portal.org/smash/get/diva2:840007/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
856 | 4 | u https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0128306&type=printable |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-120047 |
856 | 4 8 | u https://doi.org/10.1371/journal.pone.0128306 |
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