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Conversion of Synth...
Conversion of Synthetic A beta to In Vivo Active Seeds and Amyloid Plaque Formation in a Hippocampal Slice Culture Model
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- Novotny, Renata (författare)
- University of Tubingen, Germany; German Centre Neurodegenerat Disease, Germany; University of Tubingen, Germany
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- Langer, Franziska (författare)
- University of Tubingen, Germany; German Centre Neurodegenerat Disease, Germany
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- Mahler, Jasmin (författare)
- University of Tubingen, Germany; German Centre Neurodegenerat Disease, Germany; University of Tubingen, Germany
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- Skodras, Angelos (författare)
- University of Tubingen, Germany; German Centre Neurodegenerat Disease, Germany
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- Vlachos, Andreas (författare)
- Goethe University of Frankfurt, Germany
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- Wegenast-Braun, Bettina M. (författare)
- University of Tubingen, Germany; German Centre Neurodegenerat Disease, Germany
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- Kaeser, Stephan A. (författare)
- University of Tubingen, Germany; German Centre Neurodegenerat Disease, Germany
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- Neher, Jonas J. (författare)
- University of Tubingen, Germany; German Centre Neurodegenerat Disease, Germany
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- Eisele, Yvonne S. (författare)
- University of Tubingen, Germany; German Centre Neurodegenerat Disease, Germany
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- Pietrowski, Marie J. (författare)
- University of Freiburg, Germany
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- Nilsson, Peter (författare)
- Linköpings universitet,Kemi,Tekniska fakulteten
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- Deller, Thomas (författare)
- Goethe University of Frankfurt, Germany
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- Staufenbiel, Matthias (författare)
- University of Tubingen, Germany; German Centre Neurodegenerat Disease, Germany
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- Heimrich, Bernd (författare)
- University of Freiburg, Germany
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- Jucker, Mathias (författare)
- University of Tubingen, Germany; German Centre Neurodegenerat Disease, Germany
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(creator_code:org_t)
- SOC NEUROSCIENCE, 2016
- 2016
- Engelska.
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Ingår i: Journal of Neuroscience. - : SOC NEUROSCIENCE. - 0270-6474 .- 1529-2401. ; 36:18, s. 5084-5093
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The aggregation of amyloid-beta peptide (A beta) inbrain is an early event and hallmark of Alzheimers disease (AD). We combined the advantages of in vitro and in vivo approaches to study cerebral beta-amyloidosis by establishing a long-term hippocampal slice culture(HSC) model. While no A beta deposition was noted in untreated HSCs of postnatal A beta precursor protein transgenic (APP tg) mice, A beta deposition emerged in HSCs when cultures were treated once with brain extract from aged APP tg mice and the culture medium was continuously supplemented with synthetic A beta. Seeded A beta deposition was also observed under the same conditions in HSCs derived from wild-type or App-null mice but in no comparable way when HSCs were fixed before cultivation. Both the nature of the brain extract and the synthetic A beta species determined the conformational characteristics of HSCA beta deposition. HSCA beta deposits induced a microglia response, spine loss, and neuritic dystrophy but no obvious neuron loss. Remarkably, in contrast to in vitro aggregated synthetic A beta, homogenates of A beta deposits containing HSCs induced cerebral beta-amyloidosis upon intracerebral inoculation into young APP tg mice. Our results demonstrate that a living cellular environment promotes the seeded conversion of synthetic A beta into a potent in vivo seeding-active form.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Nyckelord
- alzheimer; amyloid; neurodegeneration; prion-like seeding; slice culture
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- ref (ämneskategori)
- art (ämneskategori)
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Novotny, Renata
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Langer, Franzisk ...
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Mahler, Jasmin
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Skodras, Angelos
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Vlachos, Andreas
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Wegenast-Braun, ...
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visa fler...
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Kaeser, Stephan ...
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Neher, Jonas J.
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Eisele, Yvonne S ...
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Pietrowski, Mari ...
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Nilsson, Peter
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Deller, Thomas
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Staufenbiel, Mat ...
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Heimrich, Bernd
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Jucker, Mathias
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visa färre...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Neurovetenskaper
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Journal of Neuro ...
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Linköpings universitet