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Cathepsin D mediate...
Cathepsin D mediates cytochrome c release and caspase activation in human fibroblast apoptosis induced by staurosporine
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- Johansson, Ann-Charlotte (författare)
- Linköpings universitet,Experimentell patologi,Hälsouniversitetet
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Steen, Håkan (författare)
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- Öllinger, Karin (författare)
- Östergötlands Läns Landsting,Linköpings universitet,Experimentell patologi,Hälsouniversitetet,Klinisk patologi och klinisk genetik
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- Roberg, Karin (författare)
- Östergötlands Läns Landsting,Linköpings universitet,Oto-Rhino-Laryngologi,Hälsouniversitetet,Öronkliniken US
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(creator_code:org_t)
- 2003-10-24
- 2003
- Engelska.
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Ingår i: Cell Death and Differentiation. - : Springer Science and Business Media LLC. - 1350-9047 .- 1476-5403. ; 10:11, s. 1253-1259
- Relaterad länk:
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http://urn.kb.se/res...
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https://www.nature.c...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- There is increasing evidence that proteases other than caspases, for example, the lysosomal cathepsins B, D and L, are involved in apoptotic cell death. In the present study, we present data that suggest a role for cathepsin D in staurosporine-induced apoptosis in human foreskin fibroblasts. Cathepsin D and cytochrome c were detected partially released to the cytosol after exposure to 0.1 µM staurosporine for 1 h. After 4 h, activation of caspase-9 and -3 was initiated and later caspase-8 activation and a decrease in full-length Bid were detected. Pretreatment of cells with the cathepsin D inhibitor, pepstatin A, prevented cytochrome c release and caspase activation, and delayed cell death. These results imply that cytosolic cathepsin D is a key mediator in staurosporine-induced apoptosis. Analysis of the relative sequence of apoptotic events indicates that, in this cell type, cathepsin D acts upstream of cytochrome c release and caspase activation.
Nyckelord
- apoptosis
- caspases
- cathepsin D
- cytochrome c
- fibroblasts
- lysosomes
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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