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Role of protein kin...
Role of protein kinase C α for uptake of unopsonized prey and phagosomal maturation in macrophages
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- Holm, Åsa (författare)
- Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet
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- Tejle, Katarina (författare)
- Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet
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- Gunnarsson, T. (författare)
- Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet
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- Magnusson, Karl-Eric (författare)
- Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet
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- Descoteaux, A. (författare)
- INRS—Institut Armand-Frappier, Université du Québec, Laval, Qué., Canada
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- Rasmusson, Birgitta (författare)
- Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet
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(creator_code:org_t)
- 2003
- 2003
- Engelska.
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Ingår i: Biochemical and Biophysical Research Communications - BBRC. - 0006-291X .- 1090-2104. ; 302:4, s. 653-658
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Protein kinase C α (PKCα) participates in F-actin remodeling during phagocytosis and phagosomal maturation in macrophages. Leishmania donovani promastigotes, which inhibit phagosomal maturation, cause accumulation of periphagosomal F-actin instead of the dissassembly observed around other prey [Cell. Microbiol. 7 (2001) 439]. This accumulation is induced by promastigote lipophosphoglycan (LPG), which has several effects on macrophages including inhibition of PKCα. To investigate a possible connection between PKCα and LPG’s effects on actin dynamics, we utilized RAW264.7 macrophages overexpressing dominant-negative PKCα (DN PKCα). We found increased cortical F-actin and decreased phagocytic capacity, as well as defective periphagosomal F-actin breakdown and inhibited phagosomal maturation in the DN PKCα-overexpressing cells, effects similar to those seen in controls subjected to LPG-coated prey. The results indicate that PKCα is involved in F-actin turnover in macrophages and that PKCα-dependent breakdown of periphagosomal F-actin is required for phagosomal maturation, and endorse the hypothesis that intracellular survival of L. donovani involves inhibition of PKCα by LPG.
Nyckelord
- PKCα
- Actin
- Phagocytosis
- Macrophage
- Lipophosphoglycan
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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