Sökning: onr:"swepub:oai:DiVA.org:liu-156422" >
The Effect of Enzym...
The Effect of Enzymatic Digestion on Cultured Epithelial Autografts
-
- Skog, Mårten, 1982- (författare)
- Linköpings universitet,Molekylär fysik,Tekniska fakulteten
-
- Sivlér, Petter (författare)
- Linköpings universitet,Molekylär fysik,Tekniska fakulteten
-
- Steinvall, Ingrid, 1959- (författare)
- Linköpings universitet,Avdelningen för Kirurgi, Ortopedi och Onkologi,Medicinska fakulteten,Region Östergötland, Hand- och plastikkirurgiska kliniken US
-
visa fler...
-
- Aili, Daniel, 1977- (författare)
- Linköpings universitet,Molekylär fysik,Tekniska fakulteten
-
- Sjöberg, Folke, 1956- (författare)
- Linköpings universitet,Avdelningen för Kirurgi, Ortopedi och Onkologi,Medicinska fakulteten,Region Östergötland, Hand- och plastikkirurgiska kliniken US
-
- Elmasry, Moustafa, 1981- (författare)
- Linköpings universitet,Avdelningen för Kirurgi, Ortopedi och Onkologi,Medicinska fakulteten,Region Östergötland, Hand- och plastikkirurgiska kliniken US
-
visa färre...
-
(creator_code:org_t)
- 2019-04-15
- 2019
- Engelska.
-
Ingår i: Cell Transplantation. - : Sage Publications. - 0963-6897 .- 1555-3892. ; 28:5, s. 638-644
- Relaterad länk:
-
https://liu.diva-por... (primary) (Raw object)
-
visa fler...
-
https://journals.sag...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Severe burns are often treated by means of autologous skin grafts, preferably following early excision of the burnt tissue. In the case of, for example, a large surface trauma, autologous skin cells can be expanded in vitro prior to transplantation to facilitate the treatment when insufficient uninjured skin is a limitation. In this study we have analyzed the impact of the enzyme (trypsin or accutase) used for cell dissociation and the incubation time on cell viability and expansion potential, as well as expression of cell surface markers indicative of stemness. Skin was collected from five individuals undergoing abdominal reduction surgery and the epidermal compartment was digested in either trypsin or accutase. Trypsin generally generated more cells than accutase and with higher viability; however, after 7 days of subsequent culture, accutase-digested samples tended to have a higher cell count than trypsin, although the differences were not significant. No significant difference was found between the enzymes in median fluorescence intensity of the analyzed stem cell markers; however, accutase digestion generated significantly higher levels of CD117- and CD49f-positive cells, but only in the 5 h digestion group. In conclusion, digestion time appeared to affect the isolated cells more than the choice of enzyme.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- autografts
- cell culture
- epithelial cells
- keratinocytes
- stem cells
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas