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Cellular metabolism...
Cellular metabolism and pore lifetime of human skin following microprojection array mediation
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- Haridass, Isha N. (författare)
- Curtin Univ, Australia; Univ Queensland, Australia
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- Wei, Jonathan C. J. (författare)
- Univ Queensland, Australia; Delft Univ Technol, Netherlands
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- Mohammed, Yousuf H. (författare)
- Univ Queensland, Australia
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- Crichton, Michael L. (författare)
- Heriot Watt Univ, Scotland
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- Anderson, Chris (författare)
- Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten,Region Östergötland, Hudkliniken i Östergötland
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- Henricson, Joakim (författare)
- Linköpings universitet,Avdelningen för läkemedelsforskning,Medicinska fakulteten,Region Östergötland, Akutkliniken i Linköping
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- Sanchez, Washington Y. (författare)
- Univ Queensland, Australia
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- Meliga, Stefano C. (författare)
- Univ Queensland, Australia
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- Grice, Jeffrey E. (författare)
- Univ Queensland, Australia
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- Benson, Heather A. E. (författare)
- Curtin Univ, Australia
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- Kendall, Mark A. F. (författare)
- Australian Natl Univ, Australia; Univ Queensland, Australia
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- Roberts, Michael S. (författare)
- Univ Queensland, Australia; Univ South Australia, Australia
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(creator_code:org_t)
- ELSEVIER SCIENCE BV, 2019
- 2019
- Engelska.
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Ingår i: Journal of Controlled Release. - : ELSEVIER SCIENCE BV. - 0168-3659 .- 1873-4995. ; 306, s. 59-68
- Relaterad länk:
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https://espace.libra...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Skin-targeting microscale medical devices are becoming popular for therapeutic delivery and diagnosis. We used cryo-SEM, fluorescence lifetime imaging microscopy (FLIM), autofluorescence imaging microscopy and inflammatory response to study the puncturing and recovery of human skin ex vivo and in vivo after discretised puncturing by a microneedle array (Nanopatch (R)). Pores induced by the microprojections were found to close by similar to 25% in diameter within the first 30 min, and almost completely close by similar to 6 h. FLIM images of ex vivo viable epidermis showed a stable fluorescence lifetime for unpatched areas of similar to 1000 ps up to 24 h. Only the cells in the immediate puncture zones (in direct contact with projections) showed a reduction in the observed fluorescence lifetimes to between similar to 518-583 ps. The ratio of free-bound NAD(P)H (alpha 1/alpha 2) in unaffected areas of the viable epidermis was similar to 2.5-3.0, whereas the ratio at puncture holes was almost double at similar to 4.2-4.6. An exploratory pilot in vivo study also suggested similar closure rate with histamine administration to the forearms of human volunteers after Nanopatch (R) treatment, although a prolonged inflammation was observed with Tissue Viability Imaging. Overall, this work shows that the pores created by the microneedle-type medical device, Nanopatch (R), are transient, with the skin recovering rapidly within 1-2 days in the epidermis after application.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
Nyckelord
- Skin
- 3D confocal microscopy; Pore lifetime; Metabolic lifetime; Microneedles; Drug delivery; Histamine sensitisation
- FLIM
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
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Haridass, Isha N ...
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Wei, Jonathan C. ...
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Mohammed, Yousuf ...
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Crichton, Michae ...
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Anderson, Chris
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Henricson, Joaki ...
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visa fler...
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Sanchez, Washing ...
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Meliga, Stefano ...
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Grice, Jeffrey E ...
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Benson, Heather ...
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Kendall, Mark A. ...
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Roberts, Michael ...
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- MEDICIN OCH HÄLSOVETENSKAP
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Linköpings universitet