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EGOFET Peptide Apta...
EGOFET Peptide Aptasensor for Label-Free Detection of Inflammatory Cytokines in Complex Fluids
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- Berto, Marcello (författare)
- Univ Modena and Reggio Emilia, Italy; Univ Ferrara, Italy
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- Diacci, Chiara (författare)
- Univ Modena and Reggio Emilia, Italy
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- DAgata, Roberta (författare)
- Univ Catania, Italy
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- Pinti, Marcello (författare)
- Univ Modena and Reggio Emilia, Italy
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- Bianchini, Elena (författare)
- Univ Modena and Reggio Emilia, Italy
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- Di Lauro, Michele (författare)
- Univ Modena and Reggio Emilia, Italy
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- Casalini, Stefano (författare)
- Univ Modena and Reggio Emilia, Italy; Inst Ciencia Mat Barcelona ICMAB CSIC, Spain
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- Cossarizza, Andrea (författare)
- Univ Modena and Reggio Emilia, Italy
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- Berggren, Magnus (författare)
- Linköpings universitet,Laboratoriet för organisk elektronik,Tekniska fakulteten
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- Simon, Daniel (författare)
- Linköpings universitet,Laboratoriet för organisk elektronik,Tekniska fakulteten
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- Spoto, Giuseppe (författare)
- Univ Catania, Italy; Univ Catania, Italy
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- Biscarini, Fabio (författare)
- Univ Modena and Reggio Emilia, Italy
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- Bortolotti, Carlo A. (författare)
- Univ Modena and Reggio Emilia, Italy
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(creator_code:org_t)
- 2017-12-04
- 2018
- Engelska.
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Ingår i: ADVANCED BIOSYSTEMS. - : WILEY-V C H VERLAG GMBH. - 2366-7478. ; 2:2
- Relaterad länk:
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https://liu.diva-por... (primary) (Raw object)
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http://liu.diva-port...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Organic electronic transistors are rapidly emerging as ultrahigh sensitive label-free biosensors suited for point-of-care or in-field deployed applications. Most organic biosensors reported to date are based on immunorecognition between the relevant biomarkers and the immobilized antibodies, whose use is hindered by large dimensions, poor control of sequence, and relative instability. Here, an electrolyte-gated organic field effect transistor (EGOFET) biosensor where the recognition units are surface immobilized peptide aptamers (Affimer proteins) instead of antibodies is reported. Peptide aptasensor for the detection of the pro-inflammatory cytokine tumor necrosis factor alpha (TNF alpha) with a 1 x 10(-12) M limit of detection is demonstrated. Ultralow sensitivity is met even in complex solutions such as cell culture media containing 10% serum, demonstrating the remarkable ligand specificity of the device. The device performances, together with the simple one-step immobilization strategy of the recognition moieties and the low operational voltages, all prompt EGOFET peptide aptasensors as candidates for early diagnostics and monitoring at the point-of-care.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Nyckelord
- Affimer protein; binding constant; organic bioelectronics; peptide aptamers; TNF alpha
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Berto, Marcello
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Diacci, Chiara
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DAgata, Roberta
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Pinti, Marcello
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Bianchini, Elena
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Di Lauro, Michel ...
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Casalini, Stefan ...
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Cossarizza, Andr ...
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Berggren, Magnus
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Simon, Daniel
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Spoto, Giuseppe
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Biscarini, Fabio
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Bortolotti, Carl ...
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
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och Analytisk kemi
- Artiklar i publikationen
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ADVANCED BIOSYST ...
- Av lärosätet
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Linköpings universitet