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Cell Seeding Techno...
Cell Seeding Technology for Microarray-Based Quantitative Human Primary Skeletal Muscle Cell Analysis
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- Shin, Hyun Young (författare)
- DGIST, South Korea
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- Lee, Seung Joon (författare)
- DGIST, South Korea
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- Seo, Hyung Woo (författare)
- DGIST, South Korea
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- Kim, Min Young (författare)
- DGIST, South Korea
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- Intisar, Aseer (författare)
- DGIST, South Korea
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- Yea, Kyungmoo (författare)
- DGIST, South Korea
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- Cho, Sung Chun (författare)
- DGIST, South Korea
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- Lee, Yun-Il (författare)
- DGIST, South Korea
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- Kim, Young Zoon (författare)
- Sungkyunkwan Univ, South Korea; Sungkyunkwan Univ, South Korea
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- Gurel, Ogan (författare)
- DGIST, South Korea
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- van Noort, Danny (författare)
- Linköpings universitet,Teknisk biologi,Tekniska fakulteten,DGIST, South Korea
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- Park, Sang Chul (författare)
- DGIST, South Korea
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- Kim, Minseok S. (författare)
- DGIST, South Korea; DGIST, South Korea
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(creator_code:org_t)
- 2019-10-21
- 2019
- Engelska.
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Ingår i: Analytical Chemistry. - : AMER CHEMICAL SOC. - 0003-2700 .- 1520-6882. ; 91:22, s. 14214-14219
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Pipetting techniques play a crucial role in obtaining reproducible and reliable results, especially when seeding cells on small target areas, such as on microarrays, biochips or microfabricated cell culture systems. For very rare cells, such as human primary skeletal muscle cells (skMCs), manual (freehand) cell seeding techniques invariably result in nonuniform cell spreading and heterogeneous cell densities, giving rise to undesirable variations in myogenesis and differentiation. To prevent such technique-dependent variation, we have designed and fabricated a simple, low-cost pipet guidance device (PGD), and holder that works with hand-held pipettes. This work validates the accuracy and reproducibility of the PGD platform and compares its effectiveness with manual and robotic seeding techniques. The PGD system ensures reproducibility of cell seeding, comparable to that of more expensive robotic dispensing systems, resulting in a high degree of cell uniformity and homogeneous cell densities, while also enabling cell community studies. As compared to freehand pipetting, PGD-assisted seeding of C2C12 mouse myoblasts showed 5.3 times more myotube formation and likewise myotubes derived from PGD-seeded human primary skMCs were 3.6 times thicker and 2.2 times longer. These results show that this novel, yet simple PGD-assisted pipetting technique provides precise cell seeding on small targets, ensuring reproducible and reliable high-throughput cell assays.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Shin, Hyun Young
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Lee, Seung Joon
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Seo, Hyung Woo
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Kim, Min Young
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Intisar, Aseer
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Yea, Kyungmoo
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visa fler...
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Cho, Sung Chun
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Lee, Yun-Il
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Kim, Young Zoon
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Gurel, Ogan
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van Noort, Danny
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Park, Sang Chul
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Kim, Minseok S.
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Cell och molekyl ...
- Artiklar i publikationen
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Analytical Chemi ...
- Av lärosätet
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Linköpings universitet