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DNA profiles obtained from urine in snow
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- Dufva, Charlotte (författare)
- National Forensic Centre, Sweden
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- Karlsson, Emilia (författare)
- National Forensic Centre, Sweden
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- Forsberg, Christina (författare)
- National Forensic Centre, Sweden
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- (creator_code:org_t)
- ELSEVIER IRELAND LTD, 2019
- 2019
- Engelska.
- Ingår i: Forensic Science International. - : ELSEVIER IRELAND LTD. - 1875-1768 .- 1875-175X. ; 7:1, s. 544-545
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- Urine can be a potential important source of evidence when occurring at crime scenes. In case of outdoor scenes including snow a yellow colour could indicate the existence of human urine. In an effort to be able to investigate urine as a crime scene sample we have evaluated protocols for analysing DNA from urine in snow. Two different tests were performed with a smaller and a larger volume of urine dispensed on snow. The tubes were put into a freezer to mimic winter conditions. A Urine Preservative was added to some of the samples. DNA profiles were compared between samples extracted using a Urine DNA Isolation Kit and samples extracted with a Chelex-based method. In addition, a test was performed with the aim to mimic a potential crime scene. With a smaller volume of urine the best quality DNA profiles were obtained using the Urine DNA Isolation Kit without the Urine Preservative. When a larger volume of urine was handled and in the crime scene setup, there were no clear difference between the two extraction methods. Instead, the variation observed was between individuals.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Nyckelord
- Crime scene; DNA evidence; Snow; Urine
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