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Microslit on a chip...
Microslit on a chip: A simplified filter to capture circulating tumor cells enlarged with microbeads
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- Lee, Seung Joon (författare)
- Department of New Biology, DGIST, Daegu, Republic of Korea; CytoDx, Pangyo-ro, Beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea
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- Sim, Tae Seok (författare)
- Samsung Electronics, Ltd., Maetan3-dong, Youngtong-gu, Suwon-si, Gyeonggi-do, Republic of Korea
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- Shin, Hyun Young (författare)
- Department of New Biology, DGIST, Daegu, Republic of Korea
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- Lee, Jungmin (författare)
- Department of New Biology, DGIST, Daegu, Republic of Korea
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- Kim, Min Young (författare)
- Department of New Biology, DGIST, Daegu, Republic of Korea
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- Sunoo, Joseph (författare)
- CytoDx, Pangyo-ro, Beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea
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- Lee, Jeong-Gun (författare)
- Samsung Electronics, Ltd., Maetan3-dong, Youngtong-gu, Suwon-si, Gyeonggi-do, Republic of Korea
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- Yea, Kyungmoo (författare)
- Department of New Biology, DGIST, Daegu, Republic of Korea
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- Kim, Young Zoon (författare)
- Division of Neurooncology and Department of Neurosurgery, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine, Changwon, Republic of Korea
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- van Noort, Danny (författare)
- Linköpings universitet,Teknisk biologi,Tekniska fakulteten,Department of New Biology, DGIST, Daegu, Republic of Korea
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- Park, Soo Kyung (författare)
- CytoDx, Pangyo-ro, Beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea
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- Kim, Woon-Hae (författare)
- Department of New Biology, DGIST, Daegu, Republic of Korea
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- Park, Kyun Woo (författare)
- Daejeon Wellness Hospital, Beon-gil, Dongseo-daero, Daedeok-gu, Daejeon, Republic of Korea
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- Kim, Minseok S (författare)
- Department of New Biology, DGIST, Daegu, Republic of Korea; Translational Responsive Medicine Center, DGIST, Daegu, Republic of Korea
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Department of New Biology, DGIST, Daegu, Republic of Korea; CytoDx, Pangyo-ro, Beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea Samsung Electronics, Ltd, Maetan3-dong, Youngtong-gu, Suwon-si, Gyeonggi-do, Republic of Korea (creator_code:org_t)
- 2019-10-24
- 2019
- Engelska.
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Ingår i: PLOS ONE. - : Public Library of Science. - 1932-6203. ; 14:10
- Relaterad länk:
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https://liu.diva-por... (primary) (Raw object)
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https://journals.plo...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Microchips are widely used to separate circulating tumor cells (CTCs) from whole blood by virtues of sophisticated manipulation for microparticles. Here, we present a chip with an 8 µm high and 27.9 mm wide slit to capture cancer cells bound to 3 µm beads. Apart from a higher purity and recovery rate, the slit design allows for simplified fabrication, easy cell imaging, less clogging, lower chamber pressure and, therefore, higher throughput. The beads were conjugated with anti-epithelial cell adhesion molecules (anti-EpCAM) to selectively bind to breast cancer cells (MCF-7) used to spike the whole blood. The diameter of the cell-bead construct was in average 23.1 µm, making them separable from other cells in the blood. As a result, the cancer cells were separated from 5 mL of whole blood with a purity of 52.0% and a recovery rate of 91.1%, and also we confirmed that the device can be applicable to clinical samples of human breast cancer patients. The simple design with microslit, by eliminating any high-aspect ratio features, is expected to reduce possible defects on the chip and, therefore, more suitable for mass production without false separation outputs.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
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- ref (ämneskategori)
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- Av författaren/redakt...
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Lee, Seung Joon
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Sim, Tae Seok
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Shin, Hyun Young
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Lee, Jungmin
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Kim, Min Young
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Sunoo, Joseph
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visa fler...
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Lee, Jeong-Gun
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Yea, Kyungmoo
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Kim, Young Zoon
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van Noort, Danny
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Park, Soo Kyung
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Kim, Woon-Hae
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Park, Kyun Woo
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Kim, Minseok S
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visa färre...
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- MEDICIN OCH HÄLSOVETENSKAP
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Linköpings universitet