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Neuronal apoptosis ...
Neuronal apoptosis inhibitory protein : structural requirements for hippocalcin binding and effects on survival of NGF-denendent sympathetic neurons
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- Lindholm, D (författare)
- Department of Neuroscience, Neurobiology, Uppsala University, Biomedical Centre, Box 587, S-75123 Uppsala, Sweden
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- Mercer, E A (författare)
- Department of Neuroscience, Neurobiology, Uppsala University, Biomedical Centre, Box 587, S-75123 Uppsala, Sweden
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- Yu, L Y (författare)
- Institute of Biotechnology, Biocenter 1, P.O. Box 56 Viikinkaari 9, University of Helsinki, FIN-00014 Helsinki, Finland
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- Chen, Y M (författare)
- Department of Neuroscience, Neurobiology, Uppsala University, Biomedical Centre, Box 587, S-75123 Uppsala, Sweden
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- Kukkonen, J (författare)
- Department of Neuroscience, Neurobiology, Uppsala University, Biomedical Centre, Box 587, S-75123 Uppsala, Sweden
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- Korhonen, L (författare)
- Linköpings universitet,Department of Neuroscience, Neurobiology, Uppsala University, Biomedical Centre, Box 587, S-75123 Uppsala, Sweden
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- Arumae, U (författare)
- Institute of Biotechnology, Biocenter 1, P.O. Box 56 Viikinkaari 9, University of Helsinki, FIN-00014 Helsinki, Finland
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Department of Neuroscience, Neurobiology, Uppsala University, Biomedical Centre, Box 587, S-75123 Uppsala, Sweden Institute of Biotechnology, Biocenter 1, PO. Box 56 Viikinkaari 9, University of Helsinki, FIN-00014 Helsinki, Finland (creator_code:org_t)
- ELSEVIER SCIENCE BV, 2002
- 2002
- Engelska.
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Ingår i: Biochimica et Biophysica Acta - Proteins and Proteomics. - : ELSEVIER SCIENCE BV. - 1570-9639 .- 1878-1454. ; 1600:1-2, s. 138-147
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Neuronal apoptosis inhibitory protein (NAIP) has been linked to the inherited disease, spinal muscular atrophy (SMA), which occurs in children with degeneration of the motorneurons. In the nervous system, NAIP is expressed by specific classes of neurons including spinal -motorneurons. Recently, NAIP was shown to interact with hippocalcin, which belongs to the neuronal calcium sensor (NCS) protein family. Here we-have studied this interaction in more detail, using deletions and a mutagenesis of the third baculovirus inhibitory repeat (BIR) motif in NAIP, and functional assays for neuronal death. The results showed that specific amino acids and the zinc finger domain in BIR3 are needed for efficient interaction of NAIP with hippocalcin. Cotransfections of NAIP-BIR3 and hippocalcin resulted in translocation and colocalisation of the two proteins in neuroblastoma cells. This was accompanied by an enhanced resistance towards cell death induced by high levels of calcium. In contrast, expression of NAIP-BIR3 and hippocalcin in sympathetic neurons did not protect against death induced by nerve growth factor (NGF) withdrawal. The results demonstrate a functional interaction of hippocalcin with NAIP-BIR3, which in neuroblastoma cells leads to rescue of cells after high intracellular calcium, but which in sympathetic neurons had no significant effect. The results indicate that NAIP in conjunction with hippocalcin can affect the survival of some, but not all neural cells, and this interaction may play a role in the neurodegenerative processes in SMA, and possible other human disorders. (C) 2002 Elsevier Science B.V All rights reserved.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Nyckelord
- NAIP
- BIR3
- mutation
- hippocalcin
- sympathetic neuron
- cell death
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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