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Expression of c-Met...
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Korhonen, LauraUppsala University, Uppsala, Sweden
(författare)
Expression of c-Met in developing rat hippocampus : evidence for HGF as a neurotrophic factor for calbindin D-expressing neurons
- Artikel/kapitelEngelska2000
Förlag, utgivningsår, omfång ...
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2001-12-24
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Uppsala Univ, Dept Neurosci Neurobiol, S-75123 Uppsala, Sweden. Univ Kuopio, AI Virtanen Inst, FIN-70211 Kuopio, Finland. Univ Cologne, Inst Pathol, D-50924 Cologne, Germany.Wiley-Blackwell Publishing Inc.2000
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:liu-168007
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-168007URI
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https://doi.org/10.1046/j.1460-9568.2000.00260.xDOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Hepatocyte growth factor-scatter factor (HGF) is expressed in different parts of the nervous system, and has been shown to exhibit neurotrophic activity. Here we show that c-Met, the receptor for HGF, is expressed in developing rat hippocampus, with the highest levels during the first postnatal weeks. To study the function of HGF, hippocampal neurons were prepared from embryonic rats and treated with different HGF concentrations. In these cultures, HGF increased the number of neurons expressing the 28-kDa calcium-binding protein (calbindin D) in a dose-dependent manner. The effect of HGF was larger than that observed with either brain-derived neurotrophic factor (BDNF) or neurotrophin-3 (NT-3), and cotreatment of the cultures with HGF and the neurotrophins was additive with respect to calbindin D neurons. Besides affecting the number of neurons, HGF significantly increased the degree of sprouting of calbindin D-positive neurons, suggesting an influence on neuronal maturation. BDNF and NT-3 stimulated neurite outgrowth of calbindin D neurons to a much smaller degree. In contrast to calbindin D neurons, HGF did not significantly increase the number of neurons immunoreactive with the neurotransmitter gamma-aminobutyric acid (GABA) in the hippocampal cultures. Immunohistochemical studies showed that c-Met-, calbindin D- and HGF-immunoreactive cells are all present in the dentate gyrus and partly colocalize within neurons. These results show that HGF acts on calbindin D-containing hippocampal neurons and increases their neurite outgrowth, suggesting that HGF plays an important role for the maturation and function of these neurons in the hippocampus.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Sjoholm, UlrikaUppsala University, Uppsala, Sweden
(författare)
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Takei, NobuyukiUppsala University, Uppsala, Sweden
(författare)
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Kern, Michael A.Uppsala University, Uppsala, Sweden; University of Köln, Köln, Germany
(författare)
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Schirmacher, PeterUniversity of Köln, Köln, Germany
(författare)
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Castren, EeroUniversity of Kuopio. Kuopio, Finland
(författare)
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Lindholm, DanUppsala University, Uppsala, Sweden
(författare)
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Uppsala University, Uppsala, SwedenUppsala University, Uppsala, Sweden; University of Köln, Köln, Germany
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:European Journal of NeuroscienceUppsala Univ, Dept Neurosci Neurobiol, S-75123 Uppsala, Sweden. Univ Kuopio, AI Virtanen Inst, FIN-70211 Kuopio, Finland. Univ Cologne, Inst Pathol, D-50924 Cologne, Germany. : Wiley-Blackwell Publishing Inc.12:10, s. 3453-34610953-816X1460-9568
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