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Human Milk Oligosac...
Human Milk Oligosaccharides Protect against Necrotizing Enterocolitis by Activating Intestinal Cell Differentiation
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- Li, Bo (författare)
- Hosp Sick Children, Canada
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- Wu, Richard You (författare)
- Hosp Sick Children, Canada; Hosp Sick Children, Canada
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- Horne, Rachael G. (författare)
- Hosp Sick Children, Canada
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- Ahmed, Abdalla (författare)
- Hosp Sick Children, Canada; Univ Toronto, Canada
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- Lee, Dorothy (författare)
- Hosp Sick Children, Canada; Univ Toronto, Canada
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- Robinson, Shaiya C. (författare)
- Hosp Sick Children, Canada
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- Zhu, Haitao (författare)
- Hosp Sick Children, Canada; Fudan Univ, Peoples R China
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- Lee, Carol (författare)
- Hosp Sick Children, Canada
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- Cadete, Marissa (författare)
- Hosp Sick Children, Canada
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- Johnson-Henry, Kathene C. (författare)
- Hosp Sick Children, Canada
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- Landberg, Eva (författare)
- Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten,Region Östergötland, Klinisk kemi
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- Alganabi, Mashriq (författare)
- Hosp Sick Children, Canada
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- Abrahamsson, Thomas (författare)
- Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten,Region Östergötland, H.K.H. Kronprinsessan Victorias barn- och ungdomssjukhus
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- Delgado-Olguin, Paul (författare)
- Hosp Sick Children, Canada; Univ Toronto, Canada
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- Pierro, Agostino (författare)
- Hosp Sick Children, Canada
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- Sherman, Philip M. (författare)
- Hosp Sick Children, Canada
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(creator_code:org_t)
- 2020-10-04
- 2020
- Engelska.
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Ingår i: Molecular Nutrition & Food Research. - : WILEY. - 1613-4125 .- 1613-4133. ; 64:21
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Scope Necrotizing enterocolitis (NEC) is a devastating gastrointestinal emergency and currently the leading cause of mortality in preterm infants. Recent studies show that human milk oligosaccharides (HMOs) reduce the frequency and incidence of NEC; however, the molecular mechanisms for their protection are largely unexplored. Methods and results To address this gap, a genome-wide profiling of the intestinal epithelial transcriptome in response to HMOs using RNA-sequencing is performed. It is found that HMOs alter the host transcriptome in 225 unique target genes pertaining to cell proliferation and differentiation, including upregulation of stem cell differentiation marker HMGCS2. To validate these results, differentiation in Caco-2Bbe1 (Caco-2) intestinal cells is verified by Alcian Blue staining and transepithelial electrical resistance (TER) recordings. Furthermore, an in vivo model of NEC is also employed whereby neonatal pups are gavage fed HMOs. Interestingly, HMOs-fed pups show enhanced cell MUC2 differentiation and HMGCS2 expression. Conclusions These findings demonstrate HMOs protect against NEC in part by altering the differentiation of the crypt-villus axis. In addition, this study suggests that pooled HMOs directly induce a series of biological processes, which provide mechanistic insights to how HMOs protect the host intestine.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- human milk oligosaccharides; intestinal cell differentiation; necrotizing enterocolitis; proliferation
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Li, Bo
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Wu, Richard You
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Horne, Rachael G ...
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Ahmed, Abdalla
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Lee, Dorothy
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Robinson, Shaiya ...
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visa fler...
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Zhu, Haitao
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Lee, Carol
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Cadete, Marissa
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Johnson-Henry, K ...
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Landberg, Eva
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Alganabi, Mashri ...
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Abrahamsson, Tho ...
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Delgado-Olguin, ...
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Pierro, Agostino
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Sherman, Philip ...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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Molecular Nutrit ...
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Linköpings universitet