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Measurable Cytokine...
Measurable Cytokine Concentrations in Pig Seminal Plasma Are Modified by Semen Handling and Storage
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- Padilla, Lorena (författare)
- Univ Murcia, Spain
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- Barranco, Isabel (författare)
- Univ Murcia, Spain; Univ Girona, Spain
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- Parrilla, Inmaculada (författare)
- Univ Murcia, Spain
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- Lucas, Xiomara (författare)
- Univ Murcia, Spain
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- Rodriguez-Martinez, Heriberto (författare)
- Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten
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- Roca, Jordi (författare)
- Univ Murcia, Spain
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(creator_code:org_t)
- 2020-09-07
- 2020
- Engelska.
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Ingår i: Biology. - : MDPI. - 2079-7737. ; 9:9
- Relaterad länk:
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https://liu.diva-por... (primary) (Raw object)
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https://www.mdpi.com...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Sample handling and storing are critical steps for the reliable measurement of circulating biomolecules in biological fluids. This study evaluates how cytokine measurements in pig seminal plasma (SP) vary depending on semen handling and SP storage. Thirteen cytokines (GM-CSF, IFN gamma, IL-1 alpha, IL-1 beta, IL-1ra, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-18 and TNF alpha) were measured using Luminex xMAP(R) technology in individual seminal plasma (SP) samples (n = 62) from healthy breeding boars. Three separate experiments explored the delay (2 h and 24 h) in SP collection after ejaculation (Experiment 1) and SP storage, either short-term (5 degrees C, -20 degrees C and -80 degrees C for 72 h, Experiment 2) or long-term (at -20 degrees C and -80 degrees C for two months, Experiment 3), before analysis. Levels in fresh SP-samples were used as baseline control values. Delays in SP harvesting of up to 24 h did not substantially impact SP cytokine measurements. Some cytokines showed instability in stored SP samples, mainly in long-term storage. Ideally, cytokines in pig SP should be measured in fresh samples harvested within 24 h after ejaculation. If storage of SP is imperative, storage conditions should be adjusted for each cytokine.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Nyckelord
- seminal plasma; storage; cytokines; pig
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Biology
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