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Clinical and immuno...
Clinical and immunological responses in sheep after inoculation with Himar1-transformed Anaplasma phagocytophilum and subsequent challenge with a virulent strain of the bacterium
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- Eskeland, Sveinung (författare)
- Norwegian Univ Life Sci, Norway
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- Stuen, Snorre (författare)
- Norwegian Univ Life Sci, Norway
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- Munderloh, Ulrike G. (författare)
- Univ Minnesota, MN 55108 USA
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- Barbet, Anthony (författare)
- Univ Florida, FL 32608 USA
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- Crosby, Liliana (författare)
- Univ Florida, FL 32608 USA
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- Lybeck, Kari (författare)
- Norwegian Vet Inst, Norway
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- Wilhelmsson, Peter (författare)
- Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten,Cty Hosp Ryhov, Sweden
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- Lindgren, Per-Eric (författare)
- Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten,Cty Hosp Ryhov, Sweden
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- Makvandi-Nejad, Shokouh (författare)
- Norwegian Vet Inst, Norway
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- Tollefsen, Stig (författare)
- Norwegian Vet Inst, Norway; Coalit Epidem Preparedness CEPI, Norway
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- Granquist, Erik G. (författare)
- Norwegian Univ Life Sci, Norway
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(creator_code:org_t)
- ELSEVIER, 2021
- 2021
- Engelska.
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Ingår i: Veterinary Immunology and Immunopathology. - : ELSEVIER. - 0165-2427 .- 1873-2534. ; 231
- Relaterad länk:
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https://liu.diva-por... (primary) (Raw object)
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- In Norway, the tick-transmitted bacterium Anaplasma phagocytophilum is estimated to cause tick-borne fever (TBF) in 300 000 lambs on pastures each year, resulting in economic and animal welfare consequences. Today, prophylactic measures mainly involve the use of acaricides, but a vaccine has been requested by farmers and veterinarians for decades. Several attempts have been made to produce a vaccine against A. phagocytophilum including antigenic surface proteins, inactivated whole cell vaccines and challenge followed by treatment. In the current study, a virulent wild type strain of A. phagocytophilum named Ap.Norvar1 (16S rRNA sequence partial identical to sequence in GenBank acc.no M73220) was subject to genetic transformation with a Himar1-transposon, which resulted in three bacterial mutants, capable of propagation in a tick cell line (ISE6). In order to test the immunogenicity and pathogenicity of the live, mutated bacteria, these were clinically tested in an inoculation- and challenge study in sheep. One group was inoculated with the Ap.Norvar1 as an infection control. After inoculation, the sheep inoculated with mutated bacteria and the Ap.Norvar1 developed typical clinical signs of infection and humoral immune response. After challenge with Ap.Norvar1, 28 days later all groups inoculated with mutated bacteria showed clinical signs of tick-borne fever and bacteremia while the group initially inoculated with the Ap.Norvar1, showed protection against clinical disease. The current study shows a weak, but partial protection against infection in animals inoculated with mutated bacteria, while animals that received Ap.Norvar1 both for inoculation and challenge, responded with homologues protection.
Ämnesord
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
Nyckelord
- Anaplasma phagocytophilum; Himar1-mutants; Vaccination; Serological response; Peripheral blood mononuclear cells
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Eskeland, Sveinu ...
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Stuen, Snorre
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Munderloh, Ulrik ...
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Barbet, Anthony
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Crosby, Liliana
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Lybeck, Kari
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visa fler...
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Wilhelmsson, Pet ...
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Lindgren, Per-Er ...
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Makvandi-Nejad, ...
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Tollefsen, Stig
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Granquist, Erik ...
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visa färre...
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- NATURVETENSKAP
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NATURVETENSKAP
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Veterinary Immun ...
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