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A cell-based sensor...
A cell-based sensor system for toxicity testing using multiwavelength fluorescence spectroscopy
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- Fritzsche, Michael (författare)
- Linköpings universitet,Teknisk biologi,Tekniska fakulteten
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- Fredriksson, Magnus (författare)
- Linköpings universitet,Teknisk biologi,Tekniska fakulteten
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- Carlsson, Maria (författare)
- Linköpings universitet,Teknisk biologi,Tekniska högskolan
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- Mandenius, Carl-Fredrik (författare)
- Linköpings universitet,Teknisk biologi,Tekniska högskolan
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(creator_code:org_t)
- Elsevier BV, 2009
- 2009
- Engelska.
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Ingår i: ANALYTICAL BIOCHEMISTRY. - : Elsevier BV. - 0003-2697. ; 387:2, s. 271-275
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- A novel cell-based fluorometric sensor system for toxicity monitoring is described, which uses functional spontaneously contracting cardiomyocytes (HL-1 cell line) as the biological recognition element. Based on these highly specialized cells, it has the potential of providing a sensitive and relevant analytical in vitro toxicity testing method. The system was configured by propagating the surface-attaching HL-1 cardiomyocytes in the wells of a 96-well microtiter plate and connecting the plate via an optical fiber to a fluorescence spectrometer capable of excitation-emission matrix scanning. The fluorescence data were analyzed using a conventional spectral analysis software program. The performance of the system for detection of general cytotoxicity to the cells was evaluated using three well-known drugs: verapamil, quinidine, and acetaminophen. The dose-response curves were assessed and the EC50 values were determined (0.10 +/- 0.007, 0.23 +/- 0.025, and 12.32 +/- 2.40 mM, respectively). Comparison with in vitro and in vivo reference data for the drugs showed good correlations, suggesting that this cell-based sensor system Could be a useful tool in pharmacological in vitro drug testing.
Nyckelord
- Autofluorescence
- HL-1 cell line
- Cardiomyocytes
- Cytotoxicity screening
- Drug testing
- Microtiter plate
- Tryptophan fluorescence
- In vitro toxicity
- TECHNOLOGY
- TEKNIKVETENSKAP
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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