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Antagonism of Adher...
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Alkaissi, Lina Y.Linköpings universitet,Medicinska fakulteten,Avdelningen för kirurgi, ortopedi och onkologi
(författare)
Antagonism of Adherent Invasive E. coli LF82 With Human α-defensin 5 in the Follicle-associated Epithelium of Patients With Ileal Crohn’s Disease
- Artikel/kapitelEngelska2021
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2020-12-18
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OXFORD UNIV PRESS INC,2021
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LIBRIS-ID:oai:DiVA.org:liu-177745
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-177745URI
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https://doi.org/10.1093/ibd/izaa315DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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Funding: International Organization For the Study of Inflammatory Bowel Disease (IOIBD); Lions Clubs International Foundation; Swedish Research CouncilSwedish Research CouncilEuropean Commission [2014-02537, 2017-02475]; ALF Grants Region Ostergotland
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Background: The first visible signs of Crohns disease (CD) are microscopic erosions over the follicle-associated epithelium (FAE). The aim of the study was to investigate the effects of human alpha-defensin 5 (HD5) on adherent-invasive Escherichia coli LF82 translocation and HD5 secretion after LF82 exposure in an in vitro model of human FAE and in human FAE ex vivo. Methods: An in vitro FAE-model was set up by the coculture of Raji B cells and Caco-2-cl1 cells. Ileal FAE from patients with CD and controls were mounted in Ussing chambers. The effect of HD5 on LF82 translocation was studied by LF82 exposure to the cells or tissues with or without incubation with HD5. The HD5 secretion was measured in human FAE exposed to LF82 or Salmonella typhimurium. The HD5 levels were evaluated by immunofluorescence, immunoblotting, and ELISA. Results: There was an increased LF82 translocation across the FAE-model compared with Caco-2-cl1 (P < 0.05). Incubation of cell/tissues with HD5 before LF82 exposure reduced bacterial passage in both models. Human FAE showed increased LF82 translocation in CD compared with controls and attenuated passage after incubation with sublethal HD5 in both CD and controls (P < 0.05). LF82 exposure resulted in a lower HD5 secretion in CD FAE compared with controls (P < 0.05), whereas Salmonella exposure caused equal secretion on CD and controls. There were significantly lower HD5 levels in CD tissues compared with controls. Conclusions: Sublethal HD5 reduces the ability of LF82 to translocate through FAE. The HD5 is secreted less in CD in response to LF82, despite a normal response to Salmonella. This further implicates the integrated role of antimicrobial factors and barrier function in CD pathogenesis.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Winberg Tinnerfelt, Martin,1976-Linköpings universitet,Avdelningen för kirurgi, ortopedi och onkologi,Medicinska fakulteten(Swepub:liu)marwi07
(författare)
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Heil, StéphanieLinköpings universitet,Avdelningen för molekylär medicin och virologi,Medicinska fakulteten(Swepub:liu)steda29
(författare)
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Haapaniemi, StaffanLinköpings universitet,Institutionen för biomedicinska och kliniska vetenskaper,Medicinska fakulteten,Region Östergötland, Kirurgiska kliniken ViN(Swepub:liu)staha71
(författare)
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Myrelid, Pär,1970-Linköpings universitet,Avdelningen för kirurgi, ortopedi och onkologi,Medicinska fakulteten,Region Östergötland, Kirurgiska kliniken US(Swepub:liu)parmy24
(författare)
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Stange, Eduard FDepartment of Gastroenterology, Dept. Internal Medicine I, University of Tübingen, 72076 Tübingen, Germany
(författare)
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Söderholm, Johan D,1958-Linköpings universitet,Avdelningen för kirurgi, ortopedi och onkologi,Medicinska fakulteten,Region Östergötland, Kirurgiska kliniken US(Swepub:liu)sodda63
(författare)
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Keita, Åsa,1973-Linköpings universitet,Avdelningen för kirurgi, ortopedi och onkologi,Medicinska fakulteten(Swepub:liu)asave24
(författare)
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Linköpings universitetMedicinska fakulteten
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Inflammatory Bowel Diseases: OXFORD UNIV PRESS INC27:7, s. 1116-11271078-09981536-4844
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