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Improved functional...
Improved functionality of Ligilactobacillus salivarius Li01 in alleviating colonic inflammation by layer-by-layer microencapsulation
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- Yao, Mingfei (författare)
- Zhejiang Univ, Peoples R China
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- Lu, Yanmeng (författare)
- Zhejiang Univ, Peoples R China
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- Zhang, Ting (författare)
- Zhejiang Univ, Peoples R China
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visa fler...
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- Xie, Jiaojiao (författare)
- Zhejiang Univ, Peoples R China
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- Han, Shengyi (författare)
- Zhejiang Univ, Peoples R China
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- Zhang, Shuobo (författare)
- Zhejiang Univ, Peoples R China
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- Fei, Yiqiu (författare)
- Zhejiang Univ, Peoples R China
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- Ling, Zongxin (författare)
- Zhejiang Univ, Peoples R China
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- Wu, Jingjing (författare)
- Zhejiang Univ, Peoples R China
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- Hu, Yue (författare)
- Zhejiang Univ, Peoples R China
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- Ji, Shouling (författare)
- Zhejiang Univ, Peoples R China
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- Chen, Hao (författare)
- Chinese Acad Sci, Peoples R China
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- Berglund, Björn (författare)
- Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten,Zhejiang Univ, Peoples R China
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- Li, Lanjuan (författare)
- Zhejiang Univ, Peoples R China
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(creator_code:org_t)
- 2021-07-09
- 2021
- Engelska.
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Ingår i: npj Biofilms and Microbiomes. - : Nature Research. - 2055-5008. ; 7:1
- Relaterad länk:
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https://liu.diva-por... (primary) (Raw object)
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https://www.nature.c...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The low viability during gastrointestinal transit and poor mucoadhesion considerably limits the effectiveness of Ligilactobacillus salivarius Li01 (Li01) in regulating gut microbiota and alleviating inflammatory bowel disease (IBD). In this study, a delivery system was designed through layer-by-layer (LbL) encapsulating a single Li01cell with chitosan and alginate. The layers were strengthened by cross-linking to form a firm and mucoadhesive shell (similar to 10 nm thickness) covering the bacterial cell. The LbL Li01 displayed improved viability under simulated gastrointestinal conditions and mucoadhesive function. Almost no cells could be detected among the free Li01 after 2 h incubation in digestive fluids, while for LbL Li01, the total reduction was around 3 log CFU/mL and the viable number of cells remained above 6 log CFU/mL. Besides, a 5-fold increase in the value of rupture length and a two-fold increase in the number of peaks were found in the (bacteria-mucin) adhesion curves of LbL Li01, compared to those of free Li01. Oral administration with LbL Li01 on colitis mice facilitated intestinal barrier recovery and restoration of the gut microbiota. The improved functionality of Li01 by LbL encapsulation could increase the potential for the probiotic to be used in clinical applications to treat IBD; this should be explored in future studies.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Yao, Mingfei
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Lu, Yanmeng
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Zhang, Ting
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Xie, Jiaojiao
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Han, Shengyi
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Zhang, Shuobo
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visa fler...
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Fei, Yiqiu
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Ling, Zongxin
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Wu, Jingjing
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Hu, Yue
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Ji, Shouling
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Chen, Hao
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Berglund, Björn
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Li, Lanjuan
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Immunologi inom ...
- Artiklar i publikationen
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npj Biofilms and ...
- Av lärosätet
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Linköpings universitet