Sökning: onr:"swepub:oai:DiVA.org:liu-182515" >
Comparison of Sampl...
Comparison of Sample Preparation Methods for Shotgun Proteomic Studies in Aquaculture Species
-
- Araujo, Mario Jorge (författare)
- Univ Porto, Portugal
-
- Sousa, Maria Ligia (författare)
- Univ Porto, Portugal
-
- Felpeto, Aldo Barreiro (författare)
- Univ Porto, Portugal
-
visa fler...
-
- Turkina, Maria V, 1973- (författare)
- Linköpings universitet,Avdelningen för neurobiologi,Medicinska fakulteten
-
- Fonseca, Elza (författare)
- Univ Porto, Portugal
-
- Martins, Jose Carlos (författare)
- Univ Porto, Portugal
-
- Vasconcelos, Vitor (författare)
- Univ Porto, Portugal; Univ Porto, Portugal
-
- Campos, Alexandre (författare)
- Univ Porto, Portugal
-
visa färre...
-
(creator_code:org_t)
- 2021-11-16
- 2021
- Engelska.
-
Ingår i: Proteomes. - : MDPI. - 2227-7382. ; 9:4
- Relaterad länk:
-
https://liu.diva-por... (primary) (Raw object)
-
visa fler...
-
https://doi.org/10.3...
-
https://urn.kb.se/re...
-
https://doi.org/10.3...
-
visa färre...
Abstract
Ämnesord
Stäng
- Proteomics has been recently introduced in aquaculture research, and more methodological studies are needed to improve the quality of proteomics studies. Therefore, this work aims to compare three sample preparation methods for shotgun LC-MS/MS proteomics using tissues of two aquaculture species: liver of turbot Scophthalmus maximus and hepatopancreas of Mediterranean mussel Mytilus galloprovincialis. We compared the three most common sample preparation workflows for shotgun analysis: filter-aided sample preparation (FASP), suspension-trapping (S-Trap), and solid-phase-enhanced sample preparations (SP3). FASP showed the highest number of protein identifications for turbot samples, and S-Trap outperformed other methods for mussel samples. Subsequent functional analysis revealed a large number of Gene Ontology (GO) terms in turbot liver proteins (nearly 300 GO terms), while fewer GOs were found in mussel proteins (nearly 150 GO terms for FASP and S-Trap and 107 for SP3). This result may reflect the poor annotation of the genomic information in this specific group of animals. FASP was confirmed as the most consistent method for shotgun proteomic studies; however, the use of the other two methods might be important in specific experimental conditions (e.g., when samples have a very low amount of protein).
Ämnesord
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
Nyckelord
- aquaculture; Mytilus galloprovincialis; Scophthalmus maximus; protein profiling; functional analysis; FASP; SP3; S-Trap
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas