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Comparison of SARS-...
Comparison of SARS-CoV-2 spike-specific IgA and IgG in nasal secretions, saliva and serum
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- Bladh, Oscar (författare)
- Danderyd Hosp, Karolinska Inst, Dept Clin Sci, Stockholm, Sweden.
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- Aguilera, Katherina (författare)
- Danderyd Hosp, Karolinska Inst, Dept Clin Sci, Stockholm, Sweden.
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- Marking, Ulrika (författare)
- Danderyd Hosp, Karolinska Inst, Dept Clin Sci, Stockholm, Sweden.;Publ Hlth Agcy Sweden, Solna, Sweden.
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- Kihlgren, Martha (författare)
- Danderyd Hosp, Karolinska Inst, Dept Clin Sci, Stockholm, Sweden.
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- Norin, Nina Greilert (författare)
- Danderyd Hosp, Karolinska Inst, Dept Clin Sci, Stockholm, Sweden.
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- Smed-Sorensen, Anna (författare)
- Karolinska Institutet
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- Chen, Margaret Sallberg (författare)
- Karolinska Institutet
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- Klingström, Jonas (författare)
- Linköpings universitet,Avdelningen för molekylär medicin och virologi,Medicinska fakulteten,Publ Hlth Agcy Sweden, Solna, Sweden.;Linköping Univ, Dept Biomed & Clin Sci BKV, Linköping, Sweden.
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- Blom, Kim (författare)
- Danderyd Hosp, Karolinska Inst, Dept Clin Sci, Stockholm, Sweden.;Publ Hlth Agcy Sweden, Solna, Sweden.
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- Russell, Michael W. (författare)
- Univ Buffalo, Jacobs Sch Med & Biomed Sci, Dept Microbiol & Immunol, Buffalo, NY USA.
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- Havervall, Sebastian (författare)
- Karolinska Institutet
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- Thalin, Charlotte (författare)
- Karolinska Institutet
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- Åberg, Mikael (författare)
- Uppsala universitet,Klinisk kemi,Science for Life Laboratory, SciLifeLab,Uppsala Univ, Sweden
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Danderyd Hosp, Karolinska Inst, Dept Clin Sci, Stockholm, Sweden Danderyd Hosp, Karolinska Inst, Dept Clin Sci, Stockholm, Sweden.;Publ Hlth Agcy Sweden, Solna, Sweden. (creator_code:org_t)
- Frontiers Media S.A. 2024
- 2024
- Engelska.
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Ingår i: Frontiers in Immunology. - : Frontiers Media S.A.. - 1664-3224. ; 15
- Relaterad länk:
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https://doi.org/10.3...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.3...
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https://urn.kb.se/re...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Introduction: Several novel vaccine platforms aim at mucosal immunity in the respiratory tract to block SARS-CoV-2 transmission. Standardized methods for mucosal sample collection and quantification of mucosal antibodies are therefore urgently needed for harmonized comparisons and interpretations across mucosal vaccine trials and real-world data.Methods: Using commercial electrochemiluminescence antibody panels, we compared SARS-CoV-2 spike-specific IgA and IgG in paired saliva, nasal secretions, and serum from 1048 healthcare workers with and without prior infection.Results: Spike-specific IgA correlated well in nasal secretions and saliva (r>0.65, p<0.0001), but the levels were more than three-fold higher in nasal secretions as compared to in saliva (p<0.01). Correlations between the total population of spike-specific IgA and spike-specific secretory IgA (SIgA) were significantly stronger (p<0.0001) in nasal secretions (r=0.96, p<0.0001) as opposed to in saliva (r=0.77, p<0.0001), and spike-specific IgA correlated stronger (p<0.0001) between serum and saliva (r=0.73, p<0.001) as opposed to between serum and nasal secretions (r=0.54, p<0.001), suggesting transudation of monomeric spike specific IgA from the circulation to saliva. Notably, spike-specific SIgA had a markedly higher SARS-CoV-2 variant cross-binding capacity as compared to the total population of spike specific IgA and IgG in both nasal secretions, saliva and serum, (all p<0.0001), which emphasizes the importance of taking potential serum derived monomeric IgA into consideration when investigating mucosal immune responses.Discussion: Taken together, although spike-specific IgA can be reliably measured in both nasal secretions and saliva, our findings imply an advantage of higher levels and likely also a larger proportion of SIgA in nasal secretions as compared to in saliva. We further corroborate the superior variant cross-binding capacity of SIgA in mucosal secretions, highlighting the potential protective benefits of a vaccine targeting the upper respiratory tract.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Nyckelord
- SARS-CoV-2
- Covid-19
- vaccines
- mucosal immunity
- antibodies
- secretory IgA
- saliva sampling
- nasal sampling
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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- Av författaren/redakt...
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Bladh, Oscar
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Aguilera, Kather ...
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Marking, Ulrika
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Kihlgren, Martha
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Norin, Nina Grei ...
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Smed-Sorensen, A ...
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visa fler...
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Chen, Margaret S ...
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Klingström, Jona ...
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Blom, Kim
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Russell, Michael ...
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Havervall, Sebas ...
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Thalin, Charlott ...
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Åberg, Mikael
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Uppsala universitet
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Linköpings universitet
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