Sökning: onr:"swepub:oai:DiVA.org:liu-20992" >
7ß-hydroxycholester...
7ß-hydroxycholesterol induces natural killer cell death via oxidative lysosomal destabilization
-
- Li, Wei (författare)
- Linköpings universitet,Farmakologi,Hälsouniversitetet
-
- Johnson, Henrik (författare)
- Linköpings universitet,Experimentell patologi,Hälsouniversitetet
-
- Yuan, Xi-Ming (författare)
- Linköpings universitet,Experimentell patologi,Hälsouniversitetet
-
visa fler...
-
- Jonasson, Lena (författare)
- Östergötlands Läns Landsting,Linköpings universitet,Internmedicin,Hälsouniversitetet,Kardiologiska kliniken,Avdelningen för kardiovaskulär medicin
-
visa färre...
-
(creator_code:org_t)
- 2009-10-08
- 2009
- Engelska.
-
Ingår i: Free radical research. - : Informa UK Limited. - 1071-5762 .- 1029-2470. ; 43:11, s. 1072-1079
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
visa färre...
Abstract
Ämnesord
Stäng
- Peripheral natural killer (NK) cells are reduced in patients with coronary artery disease and highly susceptible to apoptosis induced by oxidized lipids including 7beta-hydroxycholesterol (7betaOH) in vitro. The present study aimed to further explore the mechanisms behind 7betaOH-mediated cytotoxicity to human NK cells. Human NK cells were purified and treated with 7betaOH in different concentrations and times. Cell death, lysosomal and mitochondrial permeabilization and reactive oxygen species (ROS) production were then analysed. The 7betaOH induced time and dose dependent apoptosis and necrosis in human NK cells, which was preceded by loss of lysosomal integrity and enhanced ROS production. At later time points, the mitochondrial membrane permeability in 7betaOH-treated cells was significantly increased. The findings indicate that 7betaOH induces human NK cell death through early lysosomal permeabilization and consequent oxidative stress. The data further suggest that 7betaOH may induce immune disturbances in clinical settings such as atherosclerosis.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Atherosclerosis
- cell death
- 7β-hydroxycholesterol
- lysosomal membrane destabilization
- NK cells
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas