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PLUNC in human nasa...
PLUNC in human nasal lavage fluid : multiple isoforms that bind to lipopolysaccharide
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- Ghafouri, Bijar, 1972- (författare)
- Linköpings universitet,Yrkes- och miljömedicin,Hälsouniversitetet
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- Kihlström, Erik, 1948- (författare)
- Linköpings universitet,Klinisk mikrobiologi,Hälsouniversitetet
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- Tagesson, Christer, 1948- (författare)
- Linköpings universitet,Yrkes- och miljömedicin,Hälsouniversitetet
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- Lindahl, Mats, 1957- (författare)
- Linköpings universitet,Yrkes- och miljömedicin,Hälsouniversitetet
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(creator_code:org_t)
- 2004
- 2004
- Engelska.
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Ingår i: Biochimica et Biophysica Acta - Proteins and Proteomics. - 1570-9639 .- 1878-1454. ; 1699:1-2, s. 57-63
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Here, we demonstrate the presence of multiple isoforms of palate lung nasal epithelial clone (PLUNC) in human nasal lavage fluid (NLF). Eight isoforms were separated by two-dimensional gel electrophoresis (2-DE), and peptide mapping of the proteins was performed using MALDI-TOF MS (matrix assisted laser desorption/ionization time of flight mass spectrometry) of tryptic and asparginase cleavages. The identification was verified by amino acid sequencing after analysis of collision-induced dissociation (CID) fragmentation spectra with nanoelectrospray MS/MS. One isoform showed an electrophoretic mobility shift after N-glycosidase treatment, indicating that at least one of the PLUNC isoforms is glycosylated. We also demonstrate that PLUNC in NLF binds to lipopolysaccharide (LPS) in vitro; indeed, out of all proteins present in NLF only the PLUNC isoforms were found to adsorb to an LPS-coated surface. These results show that PLUNC is expressed as multiple LPS-binding isoforms in human NLF. The possibility that PLUNC may play a role in the innate immune response of the upper airways is inferred.
Nyckelord
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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