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The influence of ni...
The influence of nitric oxide on basal and cholecystokinin-8-induced proliferation and apoptosis in the rat pancreas
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- Trulsson, Lena, 1950- (författare)
- Linköpings universitet,Kirurgi,Hälsouniversitetet
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- Gasslander, Thomas, 1952- (författare)
- Department of Surgery, Vrinnevi Hospital, Norrköping, Sweden
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- Sundqvist, Tommy, 1949- (författare)
- Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet
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- Svanvik, Joar, 1942- (författare)
- Linköpings universitet,Kirurgi,Hälsouniversitetet
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(creator_code:org_t)
- 2002
- 2002
- Engelska.
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Ingår i: Regulatory Peptides. - 0167-0115 .- 1873-1686. ; 106:1-3, s. 97-104
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Nitric oxide (NO) is formed by different cell types in the pancreas. In this study, inhibition of endogenous nitric oxide by Nω-nitro-l-arginine (l-NNA) reduced the urinary excretion of NO2/NO3 and raised serum l-arginine and the NO donator S-nitroso-N-acetylpenicillamine (SNAP) increased the urinary excretion of NO2/NO3. The peptide cholecystokinin-8 (CCK-8) has a strong influence on exocrine pancreatic proliferation. Rat pancreas was excised and studied with regard to tissue weight, protein and DNA contents after 3 days of treatment with saline, l-NNA or SNAP given separately or combined with CCK-8. Further, proliferation of different pancreatic cells was studied with [3H]-thymidine incorporation and apoptotic activity was studied by analysing caspase-3 activity and histone-associated DNA fragments. The effects of l-NNA indicate that endogenous nitric oxide formation has a tonic inhibition on apoptosis in the pancreas during both basal condition and growth stimulation by CCK-8. In CCK-induced hyperplasia, NO inhibits the proliferation of acinar cells but stimulates ductal cells. Endogenous NO may regulate the balance between proliferation and apoptosis and in a situation of growth stimulation by CCK-8, it has a tonic inhibition on both mitogenesis and apoptosis thus slowing down the acinar cell turnover in the pancreas.
Nyckelord
- MEDICINE
- MEDICIN
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- ref (ämneskategori)
- art (ämneskategori)
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