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Activation of cAMP-...
Activation of cAMP-dependent protein kinase is necessary for actin rearrangements in human neutrophils during phagocytosis
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- Ydrenius, Liselotte (författare)
- Linköpings universitet,Hälsouniversitetet,Cellbiologi
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- Majeed, Meytham, 1960- (författare)
- Linköpings universitet,Hälsouniversitetet,Medicinsk mikrobiologi
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- J Rasmusson, Birgitta, 1963- (författare)
- Linköpings universitet,Hälsouniversitetet,Medicinsk mikrobiologi
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visa fler...
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- Stendahl, Olle, 1946- (författare)
- Linköpings universitet,Hälsouniversitetet,Medicinsk mikrobiologi
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- Särndahl, Eva, 1963- (författare)
- Linköpings universitet,Hälsouniversitetet,Cellbiologi
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visa färre...
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(creator_code:org_t)
- 2000
- 2000
- Engelska.
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Ingår i: Journal of Leukocyte Biology. - 0741-5400 .- 1938-3673. ; 67:4, s. 520-528
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- We have investigated the role of cAMP and cAMP-dependent protein kinase (cAPK) in neutrophil phagocytosis. Inhibition of cAPK with H-89 reduced complement- and IgG-dependent phagocytosis to 83 and 46%, respectively. Fluorescence intensity measurements of phalloidin-stained actin in neutrophils showed a reduced amount of filamentous actin (F-actin) in pseudopods and around the phagosome in cells treated with H-89 or cAMP-elevating agents (forskolin and rolipram). The amount of phosphotyrosine-containing proteins was also reduced in pseudopods and around the phagosome. Taken together, the data show that cAMP/cAPK regulates F-actin reorganization during receptor-mediated phagocytosis, particularly triggered by IgG-FcR interaction. Our results support the hypothesis that active subcortical reorganization of F-actin is a prerequisite for FcR-mediated phagocytosis, but is less important during CR3-mediated ingestion.
Nyckelord
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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