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NSP4 Enterotoxin of...
NSP4 Enterotoxin of Rotavirus Induces Paracellular Leakage in Polarized Epithelial Cells
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- Tafazoli, Farideh, 1955- (författare)
- Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet
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- Zeng, Carl Q. (författare)
- Division of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas
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- Estes, Mary K. (författare)
- Division of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas
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- Magnusson, Karl-Eric, 1946- (författare)
- Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet
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- Svensson, Lennart (författare)
- Department of Virology, Swedish Institute for Infectious Disease Control (SMI), Karolinska Institute, Solna
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(creator_code:org_t)
- 2001
- 2001
- Engelska.
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Ingår i: Journal of Virology. - 0022-538X .- 1098-5514. ; 75:3, s. 1540-1546
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- The nonstructural NSP4 protein of rotavirus has been described as the first viral enterotoxin. In this study we have examined the effect of NSP4 on polarized epithelial cells (MDCK-1) grown on permeable filters. Apical but not basolateral administration of NSP4 was found to cause a reduction in the transepithelial electrical resistance, redistribution of filamentous actin, and an increase in paracellular passage of fluorescein isothiocyanate-dextran. Significant effects on transepithelial electrical resistance were noted after a 20- to 30-h incubation with 1 nmol of NSP4. Most surprisingly, the epithelium recovered its original integrity and electrical resistance upon removal of NSP4. Preincubation of nonconfluent MDCK-1 cells with NSP4 prevented not only development of a permeability barrier but also lateral targeting of the tight-junction-associated Zonula Occludens-1 (ZO-1) protein. Taken together, these data indicate new and specific effects of NSP4 on tight-junction biogenesis and show a novel effect of NSP4 on polarized epithelia.
Nyckelord
- MEDICINE
- MEDICIN
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- ref (ämneskategori)
- art (ämneskategori)
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