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CD4+ CD25+ regulato...
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Mjösberg, Jenny,1980-Linköpings universitet,Hälsouniversitetet,Klinisk immunologi
(författare)
CD4+ CD25+ regulatory T cells in human pregnancy : Development of a Treg-MLC-ELISPOT suppression assay and indications of paternal specific Tregs
- Artikel/kapitelEngelska2007
Förlag, utgivningsår, omfång ...
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2007-01-11
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Wiley,2007
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:liu-37719
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-37719URI
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https://doi.org/10.1111/j.1365-2567.2006.02529.xDOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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The current study was aimed at developing a one-way mixed leucocyte culture-enzyme-linked immunospot (MLC-ELISPOT) assay for the study of CD4 + CD25+ regulatory T (Treg) cells and applying this method in the study of antifetal immune reactions during human pregnancy. Twenty-one pregnant women and the corresponding fathers-to-be, and 10 non-pregnant control women and men, participated in the study. CD4+ CD25+ cells were isolated from peripheral blood mononuclear cells (PBMC) by immunomagnetic selection. Maternal/control PBMC were stimulated with paternal or unrelated PBMC in MLC. Secretion of interleukin-4 (IL-4) and interferon-γ (IFN-γ) from responder cells, with or without the presence of autologous Treg cells, was analysed by ELISPOT. PBMC from pregnant women showed increased secretion of IL-4 compared to controls. In pregnant and non-pregnant controls, Treg cells suppressed IFN-γ reactivity against paternal and unrelated alloantigens. Interestingly, T reg cells suppressed IL-4 secretion against paternal but not unrelated alloantigens during pregnancy. We have successfully developed a model for studying Treg cells in antifetal cytokine reactions during pregnancy. Results indicate that Treg cells contribute to strict regulation of both T helper type 1-like and type 2-like antifetal immune reactions. Interestingly, T helper type 2-like cells specific to unrelated alloantigens are able to escape the suppression of Treg cells, which would allow for IL-4, alongside CD4+ CD25+ Treg cells, to control potentially detrimental IFN-γ reactions during pregnancy. © 2007 Blackwell Publishing Ltd.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Berg, Göran,1946-Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Obstetrik och gynekologi,Kvinnokliniken i Linköping(Swepub:liu)gorbe09
(författare)
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Ernerudh, Jan,1952-Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Klinisk immunologi,Klinisk immunologi och transfusionsmedicin(Swepub:liu)janer15
(författare)
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Ekerfelt, Christina,1957-Linköpings universitet,Hälsouniversitetet,Klinisk immunologi(Swepub:liu)chrek55
(författare)
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Linköpings universitetHälsouniversitetet
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Immunology: Wiley120:4, s. 456-4660019-28051365-2567
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