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Sökning: onr:"swepub:oai:DiVA.org:liu-43014" > Sequence-based typi...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003489naa a2200409 4500
001oai:DiVA.org:liu-43014
003SwePub
008091010s2006 | |||||||||||000 ||eng|
009oai:prod.swepub.kib.ki.se:1932918
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-430142 URI
0247 a https://doi.org/10.1128/JCM.00003-062 DOI
0247 a http://kipublications.ki.se/Default.aspx?queryparsed=id:19329182 URI
040 a (SwePub)liud (SwePub)ki
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Hjorth, SVu Statens Serum Institut, DK-2300 Copenhagen4 aut
2451 0a Sequence-based typing of Mycoplasma genitalium reveals sexual transmission
264 1c 2006
338 a print2 rdacarrier
520 a Mycoplasma genitalium causes male nonchlamydial, nongonococcal urethritis and is associated with cervicitis and pelvic inflammatory disease in women. Epidemiological studies indicate that M. genitalium is sexually transmitted, and the aim of the present study was to further substantiate this by means of a DNA typing system. A typing assay based on a diagnostic mgpB gene PCR was developed, evaluated, and applied directly to urogenital specimens. The assay had a low limit of detection and hence a high typeability. Sequences of isolates from 52 unrelated patients were divided into 29 different sequence types, giving a discriminatory index of 0.95. Two to six M. genitalium-positive specimens were collected from each of 44 patients over a median interval of 56 days (range, 11 to 1,395). Forty had the same sequence type in consecutive specimens. Specimens collected from two men were repeatedly positive at intervals of 472 and 1,395 days, respectively, but the sequence types had changed. A new strain was introduced in one sexual dyad, and the sequence types changed subsequently. Seventy-nine M. genitalium-positive specimens from 19 couples were investigated, and all partners initially had concordant sequence types, but one couple had discordant types at one time point before a newly introduced strain took over. The present typing system is simple and reproducible and has an excellent discriminatory capacity which might prove useful in studies of sexual networks and for evaluation of treatment failures. In the laboratory, this system may document the uniqueness of newly isolated M. genitalium strains.
653 a MEDICINE
653 a MEDICIN
7001 a Björnelius, Evau Karolinska Institutet4 aut
7001 a Lidbrink, Peteru Karolinska Institutet4 aut
7001 a Falk, Lars,d 1954-u Örebro University Hospital4 aut0 (Swepub:liu)larfa68
7001 a Dohn, Bu Statens Serum Institut, DK-2300 Copenhagen4 aut
7001 a Berthelsen, Lu Statens Serum Institut, DK-2300 Copenhagen4 aut
7001 a Ma, Lu Louisiana State University Health Sciences Center4 aut
7001 a Martin, DHu Louisiana State University Health Sciences Center4 aut
7001 a Jensen, Jörgen Skovu Statens Serum Institut, DK-2300 Copenhagen4 aut
7102 a Karolinska Institutetb Statens Serum Institut, DK-2300 Copenhagen4 org
7730 t Journal of Clinical Microbiologyg 44:6, s. 2078-2083q 44:6<2078-2083x 0095-1137x 1098-660X
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-43014
8564 8u https://doi.org/10.1128/JCM.00003-06
8564 8u http://kipublications.ki.se/Default.aspx?queryparsed=id:1932918

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