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Tissue-engineered r...
Tissue-engineered recombinant human collagen-based corneal substitutes for implantation : Performance of type I versus type III collagen
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Merrett, Kimberley (författare)
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- Fagerholm, Per (författare)
- Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Oftalmiatrik,Ögonkliniken US/LiM
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- McLaughlin, Christopher R. (författare)
- Univ Ottawa, Dept Cellular & Mol Med, Ottawa, ON, Canada
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Dravida, Subhadra (författare)
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- Lagali, Neil (författare)
- University of Ottawa
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- Shinozaki, Naoshi (författare)
- Tokyo Dent Coll, Cornea Ctr & Eye Bank, Chiba, Japan
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- Watsky, Mitchell A. (författare)
- Univ Tennessee, Ctr Hlth Sci, Dept Physiol, Memphis, TN 38163 USA
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Munger, Rejean (författare)
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- Kato, Yasuhiro (författare)
- Tokyo Dent Coll, Cornea Ctr & Eye Bank, Chiba, Japan
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Li, Fengfu (författare)
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- Marmo, Christopher J. (författare)
- CooperVision Inc, Pleasanton, CA USA
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- Griffith, May (författare)
- Univ Ottawa, Dept Cellular & Mol Med, Ottawa, ON, Canada
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(creator_code:org_t)
- Association for Research in Vision and Ophthalmology (ARVO), 2008
- 2008
- Engelska.
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Ingår i: Investigative Ophthalmology and Visual Science. - : Association for Research in Vision and Ophthalmology (ARVO). - 0146-0404 .- 1552-5783. ; 49:9, s. 3887-3894
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- PURPOSE. To compare the efficacies of recombinant human collagens types I and III as corneal substitutes for implantation. METHODS. Recombinant human collagen (13.7%) type I or III was thoroughly mixed with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide. The final homogenous solution was either molded into sheets for in vitro studies or into implants with the appropriate corneal dimensions for transplantation into minipigs. Animals with implants were observed for up to 12 months after surgery. Clinical examinations of the cornea included detailed slit lamp biomicroscopy, in vivo confocal microscopy, and fundus examination. Histopathologic examinations were also performed on corneas harvested after 12 months. RESULTS. Both cross-linked recombinant collagens had refractive indices of 1.35, with optical clarity similar to that in human corneas. Their chemical and mechanical properties were similar, although RHC-III implants showed superior optical clarity. Implants into pig corneas over 12 months show comparably stable integration, with regeneration of corneal cells, tear film, and nerves. Optical clarity was also maintained in both implants, as evidenced by fundus examination. CONCLUSIONS. Both RHC-I and -III implants can be safely and stably integrated into host corneas. The simple cross-linking methodology and recombinant source of materials makes them potentially safe and effective future corneal matrix substitutes.
Nyckelord
- MEDICINE
- MEDICIN
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Merrett, Kimberl ...
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Fagerholm, Per
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McLaughlin, Chri ...
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Dravida, Subhadr ...
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Lagali, Neil
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Shinozaki, Naosh ...
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visa fler...
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Watsky, Mitchell ...
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Munger, Rejean
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Kato, Yasuhiro
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Li, Fengfu
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Marmo, Christoph ...
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Griffith, May
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Investigative Op ...
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Linköpings universitet