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Calcifying epitheli...
Calcifying epithelial odontogenic (Pindborg) tumor-associated amyloid consists of a novel human protein
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Solomon, A (författare)
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Murphy, CL (författare)
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Weaver, K (författare)
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Weiss, DT (författare)
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Hrncic, R (författare)
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Eulitz, M (författare)
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Donnell, RL (författare)
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Sletten, K (författare)
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- Westermark, Gunilla (författare)
- Linköpings universitet,Hälsouniversitetet,Cellbiologi
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Westermark, P (författare)
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(creator_code:org_t)
- 2003
- 2003
- Engelska.
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Ingår i: Journal of Laboratory and Clinical Medicine. - 0022-2143 .- 1532-6543. ; 142:5, s. 348-355
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Calcifying epithelial odontogenic tumors (CEOTs), also known as Pindborg tumors, are characterized by the presence of squamous-cell proliferation, calcification, and, notably, amyloid deposits. On the basis of immunohistochemical analyses, the amyloidogenic component had heretofore been deemed to consist of cytokeratin-related or other molecules, however, its chemical composition had never been elucidated. We have used our microanalytic techniques to characterize the protein nature of CEOT-associated amyloid isolated from specimens obtained from 3 patients. As evidenced by the results of amino-acid sequencing and mass spectrometry, the fibrils were found to be composed of a polypeptide of approximately 46 mer. This component was identical in sequence to the N-terminal portion of a hypothetical 153-residue protein encoded by the FLJ20513 gene cloned from the human KATO III cell line. That the amyloid protein was derived from this larger molecule was demonstrated by reverse transcription-polymerase chain reaction amplification of tumor-cell RNA where a full-length FLJ20513 transcript was found. Furthermore, immunohistochemical analyses revealed that the amyloid within the CEOTs immunostained with antibodies prepared against a synthetic FLJ20513-related dodecapeptide. Out studies provide unequivocal evidence that CEOT-associated amyloid consists of a unique and previously undescribed protein that we provisionally designate APin.
Nyckelord
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Solomon, A
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Murphy, CL
-
Weaver, K
-
Weiss, DT
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Hrncic, R
-
Eulitz, M
-
visa fler...
-
Donnell, RL
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Sletten, K
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Westermark, Guni ...
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Westermark, P
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visa färre...
- Artiklar i publikationen
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Journal of Labor ...
- Av lärosätet
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Linköpings universitet