Sökning: onr:"swepub:oai:DiVA.org:liu-53044" >
D variants at the R...
D variants at the RhD vestibule in the weak D type 4 and Eurasian D clusters
-
- Flegel, Willy A (författare)
- University Hospital, Ulm
-
von Zabern, Inge (författare)
-
Doescher, Andrea (författare)
-
visa fler...
-
Wagner, Franz F (författare)
-
Strathmann, Klaus P (författare)
-
Geisen, Christof (författare)
-
- Plafi, Miodrag (författare)
- Östergötlands Läns Landsting,Linköpings universitet,Transfusionsmedicin,Hälsouniversitetet,Klinisk immunologi och transfusionsmedicin,Landstinget i Östergötland
-
Pisacka, Martin (författare)
-
Poole, Joyce (författare)
-
Polin, Helene (författare)
-
Gabriel, Christian (författare)
-
Avent, Neil D (författare)
-
visa färre...
-
(creator_code:org_t)
- Wiley, 2009
- 2009
- Engelska.
-
Ingår i: Transfusion. - : Wiley. - 0041-1132 .- 1537-2995. ; 49:6, s. 1059-1069
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- BACKGROUND: One branch of the RHD phylogenetic tree is represented by the weak D type 4 cluster of alleles with F223V as the primordial amino acid substitution. F223V as well as a large number of further substitutions causing D variants are located at the extracellular RhD protein vestibule, which represents the entrance to the transmembraneous channel of the RhD protein. STUDY DESIGN AND METHODS: RHD and RHCE nucleotide sequences were determined from genomic DNA and cDNA. D epitope patterns were established with commercial monoclonal anti-D panels. RESULTS: The RHD alleles DOL-1 and DOL-2 had the two amino acid substitutions M170T (509T>C) and F223V (667T>G) in common. DOL-2 harbored the additional substitution L378V (1132C>G). Both alleles were observed in Africans and are probably evolutionary related. DMI carried M170I (510G>A), which differed from the DOL-typical substitution. DFW and DFL harbored the substitutions H166P (497A>C) and Y165C (494A>G). The antigen densities of DOL-1, DFL, and DFW were only moderately reduced. CONCLUSION: DOL-1 and DOL-2 belong to the weak D type 4 cluster of RHD alleles. Together with DMI, DFL, and DFW they represent D variants with amino acid substitutions located at extracellular loops 3 or 4 lining the RhD protein vestibule. These substitutions were of minor influence on antigen density while adjacent substitutions in the transmembraneous section caused weak D antigen expression. All these D variants were partial D and alloanti-D immunizations have been observed in DOL-1, DMI, and DFL carriers. The substitution at position 170 causes partial D although located deep in the vestibule.
Nyckelord
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Flegel, Willy A
-
von Zabern, Inge
-
Doescher, Andrea
-
Wagner, Franz F
-
Strathmann, Klau ...
-
Geisen, Christof
-
visa fler...
-
Plafi, Miodrag
-
Pisacka, Martin
-
Poole, Joyce
-
Polin, Helene
-
Gabriel, Christi ...
-
Avent, Neil D
-
visa färre...
- Artiklar i publikationen
-
Transfusion
- Av lärosätet
-
Linköpings universitet