Repartition effect of aromatic polyaniline coatings on the separation of bioactive peptides in capillary electrophoresis

• The capillary walls of fused-silica capillary electrophoresis (CE) columns were modified with a thin film of polyaniline (PANI), providing open-tubular columns with a stable coating containing aromatic groups and amine functionalities. Fast and efficient separations were observed for small bioactive peptides under acidic conditions on PANI-coated columns. The mechanism of separation is based on hydrophobic interactions between the analytes and the polymeric matrix. Good reproducibility was observed from run-to-run. Due to the simple derivatization procedure, method flexibility, the uniformity of the coating and its stability, conjugated polymers could find practical application in capillary zone electrophoresis (CZE) separations.

Nyckelord

coating; capillary electrophoresis; polyaniline; peptides; electroosmotic flow; repartition effect

TECHNOLOGY

TEKNIKVETENSKAP

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