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LACTATE, GLUTAMATE ...
LACTATE, GLUTAMATE AND GLUTAMINE BIOSENSORS BASED ON RHODINISED CARBON ELECTRODES
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- WHITE, SF (författare)
- CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,BEDFORD MK43 0AL,ENGLAND; GESELL BIOTECHNOL FORSCH MBH,W-3300 BRAUNSCHWEIG,GERMANY;
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- TURNER, APF (författare)
- Cranfield University, UK
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- BILITEWSKI, U (författare)
- CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,BEDFORD MK43 0AL,ENGLAND; GESELL BIOTECHNOL FORSCH MBH,W-3300 BRAUNSCHWEIG,GERMANY;
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- SCHMID, RD (författare)
- CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,BEDFORD MK43 0AL,ENGLAND; GESELL BIOTECHNOL FORSCH MBH,W-3300 BRAUNSCHWEIG,GERMANY;
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- BRADLEY, J (författare)
- CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,BEDFORD MK43 0AL,ENGLAND; GESELL BIOTECHNOL FORSCH MBH,W-3300 BRAUNSCHWEIG,GERMANY;
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(creator_code:org_t)
- Elsevier Science B.V., Amsterdam. 1994
- 1994
- Engelska.
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Ingår i: Analytica Chimica Acta. - : Elsevier Science B.V., Amsterdam.. - 0003-2670 .- 1873-4324. ; 295:3, s. 243-251
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- Amperometric enzyme sensors for lactate, glutamate and glutamine were constructed using rhodinised carbon electrodes. All three sensors operated at a potential of +400 mV (Ag/AgCl). Lactate sensors were constructed using lactate oxidase immobilized in hydroxyethylcellulose, overlaid with a cellulose acetate membrane. Using a flow-injection system, the sensors had a linear range of 0.1 to 1.5 mM. Measurements of lactate concentrations from a mammalian cell culture were compared with results obtained from a commercial instrument. A correlation coefficient of r=0.982 (n = 15) was obtained. Glutamate and glutamine sensors were fabricated based on glutaraldehyde immobilization; the former by incorporating glutamate oxidase and the latter based on glutamate oxidase and glutaminase (both had a linear range of 0.1 to 1.5 mM). The feasibility of using these sensors, in conjunction with a previously described glucose sensor, for mammalian cell culture monitoring is discussed
Nyckelord
- BIOSENSORS; LACTATE; GLUTAMATE; GLUTAMINE; RHODINISED CARBON ELECTRODES
- TECHNOLOGY
- TEKNIKVETENSKAP
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