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Expression of a Bro...
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Shankar, EsakimuthuLinköpings universitet,Molekylär virologi,Hälsouniversitetet
(författare)
Expression of a Broad Array of Negative Costimulatory Molecules and Blimp-1 in T Cells following Priming by HIV-1 Pulsed Dendritic Cells
- Artikel/kapitelEngelska2011
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2010-11-17
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Feinstein Institute for Medical Research,2011
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printrdacarrier
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LIBRIS-ID:oai:DiVA.org:liu-67301
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-67301URI
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https://doi.org/10.2119/molmed.2010.00175DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Accumulating evidence indicates that immune impairment in persistent viral infections could lead to T-cell exhaustion. To evaluate the potential contribution of induction of negative costimulatory molecules to impaired T-cell responses, we primed naive T cells with mature monocyte-derived dendritic cells (MDDCs) pulsed with HIV-1 in vitro. We used quantitative real-time polymerase chain reaction and flow cytometry, respectively, to compare the gene and surface-protein expression profiles of naive T cells primed with HIV-pulsed or mock-pulsed DCs. We detected elevated expressions of negative costimulatory molecules, including lymphocyte activation gene-3 (LAG-3). CD160, cytolytic T-lymphocyte antigen-4 (CTLA-4). T-cell immunoglobulin mucin-containing domain-3 (TIM-3), programmed death-1 (PD-1) and TRAIL (tumor necrosis-factor-related apoptosis-inducing ligand) in T cells primed by HIV-pulsed DCs. The PD-1(+) T-cell population also coexpressed TIM-3, LAG-3, and CTLA-4. Interestingly, we also found an increase in gene expression of the transcriptional repressors Blimp-1 (B-lymphocyte-induced maturation protein-1) and Foxp3 (forkhead transcription factor) in T-cells primed by HIV-pulsed DCs; Blimp-1 expression was directly proportional to the expression of the negative costimulatory molecules. Furthermore, levels of the effector cytokines interleukin-2, tumor necrosis factor-alpha and interferon-gamma, and perforin and granzyme B were decreased in T-cell populations primed by HIV-pulsed DCs. In conclusion, in vitro priming of halve T-cells with HIV-pulsed DC leads to expansion of T cells with coexpression of a broad array of negative costimulatory mclecules and Blimp-1, with potential deleterious consequences for T-cell responses.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Fru Che, KarlhansUniversity of California San Diego
(författare)
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Lifson, Jeffrey DNCI
(författare)
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Larsson, MarieLinköpings universitet,Molekylär virologi,Hälsouniversitetet(Swepub:liu)marla67
(författare)
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Linköpings universitetMolekylär virologi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:MOLECULAR MEDICINE: Feinstein Institute for Medical Research17:3-4, s. 229-2401076-15511528-3658
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