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Cellular level char...
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Bourghardt Peebo, BeatriceÖstergötlands Läns Landsting,Linköpings universitet,Oftalmiatrik,Hälsouniversitetet,Ögonkliniken US/LiM
(författare)
Cellular level characterization of capillary regression in inflammatory angiogenesis using an in vivo corneal model
- Artikel/kapitelEngelska2011
Förlag, utgivningsår, omfång ...
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2011-07-10
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Springer Verlag (Germany),2011
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electronicrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:liu-70325
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-70325URI
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https://doi.org/10.1007/s10456-011-9223-3DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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The original publication is available at www.springerlink.com: Beatrice Bourghardt Peebo, Per Fagerholm, Catharina Traneus-Rockert and Neil Lagali, Cellular level characterization of capillary regression in inflammatory angiogenesis using an in vivo corneal model, 2011, Angiogenesis, (14), 3, 393-405. http://dx.doi.org/10.1007/s10456-011-9223-3 Copyright: Springer Verlag (Germany) http://www.springerlink.com/
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In this study, we introduce a technique for repeated, microscopic observation of single regressing capillaries in vivo in inflamed murine corneas. Natural capillary regression was initiated by removal of inflammatory stimulus during an active pro-angiogenic phase, while the additional impact of anti-angiogenic treatment with triamcinolone or bevazicumab was investigated. Capillaries regressed naturally within 1 week and treatments did not further enhance the natural regression. Morphologically, early-phase regression was characterized by significant lumen narrowing and a significant reduction in CD11b+ myeloid cell infiltration of the extracellular matrix. By 1 week, vascular remodeling occurred concomitant with CD11b+CD68+KiM2R+ mature macrophage localization on capillary walls. Empty conduits without blood flow, positive for collagen IV and devoid of vascular endothelium and pericytes, were apparent in vivo and by 3 weeks were more numerous than perfused capillaries. By 3 weeks, macrophages aggregated around remaining perfused capillaries and were observed in apposition with degrading capillary segments. Abrupt termination of capillary sprouting in our regression model further revealed vascular endothelial abandonment of sprout tips and perfused capillary loop formation within a single angiogenic sprout, possibly as an intussusceptive response to cessation of the stimulus. Finally, we observed lumen constriction and macrophage localization on capillary walls in vivo in a clinical case of corneal capillary regression that paralleled findings in our murine model.
Ämnesord och genrebeteckningar
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Inflammation
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Capillary regression
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In vivo confocal microscopy
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Cornea
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MEDICINE
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MEDICIN
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Fagerholm, PerÖstergötlands Läns Landsting,Linköpings universitet,Oftalmiatrik,Hälsouniversitetet,Ögonkliniken US/LiM(Swepub:liu)perfa04
(författare)
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Traneus-Rockert, CatharinaÖstergötlands Läns Landsting,Klinisk patologi och klinisk genetik
(författare)
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Lagali, NeilÖstergötlands Läns Landsting,Linköpings universitet,Oftalmiatrik,Hälsouniversitetet,Ögonkliniken US/LiM(Swepub:liu)neina50
(författare)
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Linköpings universitetOftalmiatrik
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Angiogenesis: Springer Verlag (Germany)14:3, s. 393-4050969-69701573-7209
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