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Enterovirus 70 bind...
Enterovirus 70 binds to different glycoconjugates containing alpha 2,3-linked sialic acid on different cell lines
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- Nokhbeh, M. Reza (författare)
- Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Faculty of Medicine, Ottawa, ON, Canada
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- Hazra, Samir (författare)
- Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Faculty of Medicine, Ottawa, ON, Canada
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- Alexander, David A. (författare)
- Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Faculty of Medicine, Ottawa, ON, Canada
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- Khan, Ahmar (författare)
- Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Faculty of Medicine, Ottawa, ON, Canada
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- McAllister, Morgan (författare)
- Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Faculty of Medicine, Ottawa, ON, Canada
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- Suuronen, Erik J. (författare)
- Department of Cellular and Molecular Medicine, University of Ottawa, Faculty of Medicine, Ottawa, ON, Canada and University of Ottawa Eye Institute, Ottawa, ON, Canada
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- Griffith, May (författare)
- Department of Cellular and Molecular Medicine, University of Ottawa, Faculty of Medicine, Ottawa, ON, Canada and University of Ottawa Eye Institute, Ottawa, ON, Canada
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- Dimock, Kenneth (författare)
- Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Faculty of Medicine, Ottawa, ON, Canada
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(creator_code:org_t)
- American Society for Microbiology, 2005
- 2005
- Engelska.
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Ingår i: Journal of Virology. - : American Society for Microbiology. - 0022-538X .- 1098-5514. ; 79:11, s. 7087-7094
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Enterovirus 70 (EV70), the causative agent of acute hemorrhagic conjunctivitis, exhibits a restricted tropism for conjunctival and corneal cells in vivo but infects a wide spectrum of mammalian cells in culture. Previously, we demonstrated that human CD55 is a receptor for EV70 on HeLa cells but that EV70 also binds to sialic acid-containing receptors on a variety of other human cell lines. Virus recognition of sialic acid attached to underlying glycans by a particular glycosidic linkage may contribute to host range, tissue tropism, and pathogenesis. Therefore, we tested the possibility that EV70 binds to andalpha; 2,3-linked sialic acid, like other viruses associated with ocular infections. Through the use of linkage-specific sialidases, sialyltransferases, and lectins, we show that EV70 recognizes andalpha; 2,3-linked sialic acid on human corneal epithelial cells and on U-937 cells. Virus attachment to both cell lines is CD55 independent and sensitive to benzyl N-acetyl-andalpha;-D-galactosaminide, an inhibitor of O-linked glycosylation. Virus binding to corneal cells, but not U-937 cells, is inhibited by proteinase K, but not by phosphatidylinositol-specific phospholipase C treatment. These results are consistent with the idea that a major EV70 receptor on corneal epithelial cells is an O-glycosylated, non-glycosyl phosphatidylinositol-anchored membrane glycoprotein containing andalpha; 2,3-linked sialic acid, while sialylated receptors on U-937 cells are not proteinaceous.
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