Sökning: onr:"swepub:oai:DiVA.org:liu-72275" >
Gd2O3 nanoparticles...
Gd2O3 nanoparticles in hematopoietic cells for MRI contrast enhancement
-
- Hedlund, Anna (författare)
- Linköpings universitet,Medicinsk radiologi,Centrum för medicinsk bildvetenskap och visualisering, CMIV,Hälsouniversitetet
-
- Ahrén, Maria (författare)
- Linköpings universitet,Molekylär ytfysik och nanovetenskap,Tekniska fakulteten
-
- Gustafsson, Håkan, 1976- (författare)
- Linköpings universitet,Hälsouniversitetet,Medicinsk radiofysik,Centrum för medicinsk bildvetenskap och visualisering, CMIV
-
visa fler...
-
- Abrikossova, Natalia, 1965- (författare)
- Linköpings universitet,Molekylär ytfysik och nanovetenskap,Tekniska fakulteten
-
- Warntjes, Marcel, 1973- (författare)
- Linköpings universitet,Klinisk fysiologi,Hälsouniversitetet,Centrum för medicinsk bildvetenskap och visualisering, CMIV
-
- Jönsson, Jan-Ivar (författare)
- Linköpings universitet,Hälsouniversitetet,Medicinsk och fysiologisk kemi
-
- Uvdal, Kajsa (författare)
- Linköpings universitet,Molekylär ytfysik och nanovetenskap,Tekniska fakulteten
-
- Engström, Maria, 1958- (författare)
- Linköpings universitet,Medicinsk radiologi,Centrum för medicinsk bildvetenskap och visualisering, CMIV,Hälsouniversitetet
-
visa färre...
-
(creator_code:org_t)
- Manchester, UK : Dove Medical Press Ltd, 2011
- 2011
- Engelska.
-
Ingår i: International journal of nano medicine. - Manchester, UK : Dove Medical Press Ltd. - 1178-2013. ; 6, s. 3233-3240
- Relaterad länk:
-
https://liu.diva-por... (primary) (Raw object)
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.2...
-
visa färre...
Abstract
Ämnesord
Stäng
- As the utility of magnetic resonance imaging (MRI) broadens, the importance of having specific and efficient contrast agents increases and in recent time there has been a huge development in the fields of molecular imaging and intracellular markers. Previous studies have shown that gadolinium oxide (Gd2O3) nanoparticles generate higher relaxivity than currently available Gd chelates: In addition, the Gd2O3 nanoparticles have promising properties for MRI cell tracking. The aim of the present work was to study cell labeling with Gd2O3 nanoparticles in hematopoietic cells and to improve techniques for monitoring hematopoietic stem cell migration by MRI. Particle uptake was studied in two cell lines: the hematopoietic progenitor cell line Ba/F3 and the monocytic cell line THP-1. Cells were incubated with Gd2O3 nanoparticles and it was investigated whether the transfection agent protamine sulfate increased the particle uptake. Treated cells were examined by electron microscopy and MRI, and analyzed for particle content by inductively coupled plasma sector field mass spectrometry. Results showed that particles were intracellular, however, sparsely in Ba/F3. The relaxation times were shortened with increasing particle concentration. Relaxivities, r1 and r2 at 1.5 T and 21°C, for Gd2O3 nanoparticles in different cell samples were 3.6–5.3 s-1 mM-1 and 9.6–17.2 s-1 mM-1, respectively. Protamine sulfate treatment increased the uptake in both Ba/F3 cells and THP-1 cells. However, the increased uptake did not increase the relaxation rate for THP-1 as for Ba/F3, probably due to aggregation and/or saturation effects. Viability of treated cells was not significantly decreased and thus, it was concluded that the use of Gd2O3 nanoparticles is suitable for this type of cell labeling by means of detecting and monitoring hematopoietic cells. In conclusion, Gd2O3 nanoparticles are a promising material to achieve positive intracellular MRI contrast; however, further particle development needs to be performed.
Nyckelord
- gadolinium oxide
- magnetic resonance imaging
- contrast agent
- cell labeling
- Ba/F3 cells
- THP-1 cells
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas