In vitro susceptibility of CTX-M-producing Escherichia coli to non-beta-lactam agents

Östholm Balkhed, ÅseÖstergötlands Läns Landsting,Linköpings universitet,Klinisk mikrobiologi,Hälsouniversitetet (författare)

Background: The objective of this study was to investigate the in vitro activity of different antibiotics against CTX-M-producing Escherichia coli, to determine the occurrence of multiresistance and plasmid mediated quinolone resistance among these isolates. Methods: A total of 198 isolates of E. coli with ESBL phenotype and mainly CTX-M genotype, were studied. The MICs for amikacin, chloramphenicol, ciprofloxacin, colistin, fosfomycin, gentamicin, nalidixic acid, nitrofurantoin, tigecycline, tobramycin, trimethoprim and trimethoprim-sulphamethoxazole were determined with the Etest. Susceptibility was defined according to the breakpoints of the European Committee on Antimicrobial Susceptibility Testing (EUCAST). MIC(50) and MIC(90) values were calculated. Results: ≥95% of isolates were susceptible to amikacin, nitrofurantoin, colistin, tigecyclin, and fosfomycin. CTX-M group 9 was more susceptible than CTX-M group 1 to ciprofloxacin, gentamicin, and tobramycin. 68% of the isolates were multiresistant, and the most common multi-resistance pattern was ESBL-phenotype with decreased susceptibility to trimethoprim, trimethoprim-sulphamethoxazol, ciprofloxacin, gentamicin and tobramycin. Only one isolate carried a qnrS1-gene, nine isolates carried aac(6’)-Ib-cr. Conclusions: The high frequency of multi-resistance found in this study is alarming and it is urgent to find strategies to limit the emergence and spread of these multi-resistant strains.

Tärnberg, MariaLinköpings universitet,Klinisk mikrobiologi,Hälsouniversitetet(Swepub:liu)marta92 (författare)
Monstein, Hans-JurgÖstergötlands Läns Landsting,Linköpings universitet,Klinisk mikrobiologi,Hälsouniversitetet(Swepub:liu)hanmo19 (författare)
Hällgren, AnitaÖstergötlands Läns Landsting,Linköpings universitet,Infektionsmedicin,Hälsouniversitetet,Infektionskliniken i Östergötland(Swepub:liu)aniha32 (författare)
Hanberger, HåkanÖstergötlands Läns Landsting,Linköpings universitet,Infektionsmedicin,Hälsouniversitetet,Infektionskliniken i Östergötland(Swepub:liu)hakha84 (författare)
Nilsson, Lennart ELinköpings universitet,Klinisk mikrobiologi,Hälsouniversitetet(Swepub:liu)lenni43 (författare)
Linköpings universitetKlinisk mikrobiologi (creator_code:org_t)

Av författaren/redakt...: Östholm Balkhed, ...; Tärnberg, Maria; Monstein, Hans-J ...; Hällgren, Anita; Hanberger, Håkan; Nilsson, Lennart ...

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