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An Electrochemical ...
An Electrochemical Immunoassay for HER2 Detection
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- Al-Khafaji, Q A M (författare)
- University of Florence
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- Harris, M (författare)
- University of Florence
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- Tombelli, S (författare)
- University of Florence
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- Laschi, S (författare)
- University of Florence
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- Turner, Anthony (författare)
- Linköpings universitet,Biosensorer och bioelektronik,Tekniska högskolan
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- Mascini, M (författare)
- University of Florence
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- Marrazza, G (författare)
- University of Florence
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(creator_code:org_t)
- 2012-01-12
- 2012
- Engelska.
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Ingår i: Electroanalysis. - : Wiley-VCH Verlag Berlin. - 1040-0397 .- 1521-4109. ; 24:4, s. 735-742
- Relaterad länk:
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https://liu.diva-por... (primary) (Raw object)
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http://liu.diva-port...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- In this paper, a simple and sensitive approach for human epidermal growth factor receptor 2 (HER2) detection is presented, using antibody-functionalised magnetic beads coupled to screen-printed cells. The immunoassay is based on a sandwich format in which a primary monoclonal antibody anti-HER2 is coupled to protein A modified magnetic beads. The modified beads are then used to capture the protein from the sample solution and a sandwich assay is performed by adding a secondary monoclonal antibody anti-HER2 labelled with biotin. The enzyme alkaline phosphatase (AP) conjugated with streptavidin and its substrate (1-naphthyl-phosphate) are then used for the electrochemical detection by differential pulse voltammetry (DPV). The experimental conditions for the immunoassay were optimised. The performance of the assay in terms of sensitivity, reproducibility and selectivity has been studied in buffer and serum samples from hospital patients.
Nyckelord
- HER2
- Cancer marker
- Magnetic beads
- Immunoassay
- TECHNOLOGY
- TEKNIKVETENSKAP
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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