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An Electrochemical ...
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Al-Khafaji, Q A MUniversity of Florence
(författare)
An Electrochemical Immunoassay for HER2 Detection
- Artikel/kapitelEngelska2012
Förlag, utgivningsår, omfång ...
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2012-01-12
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Wiley-VCH Verlag Berlin,2012
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electronicrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:liu-76937
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-76937URI
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https://doi.org/10.1002/elan.201100501DOI
Kompletterande språkuppgifter
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Språk:engelska
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Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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In this paper, a simple and sensitive approach for human epidermal growth factor receptor 2 (HER2) detection is presented, using antibody-functionalised magnetic beads coupled to screen-printed cells. The immunoassay is based on a sandwich format in which a primary monoclonal antibody anti-HER2 is coupled to protein A modified magnetic beads. The modified beads are then used to capture the protein from the sample solution and a sandwich assay is performed by adding a secondary monoclonal antibody anti-HER2 labelled with biotin. The enzyme alkaline phosphatase (AP) conjugated with streptavidin and its substrate (1-naphthyl-phosphate) are then used for the electrochemical detection by differential pulse voltammetry (DPV). The experimental conditions for the immunoassay were optimised. The performance of the assay in terms of sensitivity, reproducibility and selectivity has been studied in buffer and serum samples from hospital patients.
Ämnesord och genrebeteckningar
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HER2
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Cancer marker
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Magnetic beads
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Immunoassay
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TECHNOLOGY
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TEKNIKVETENSKAP
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Harris, MUniversity of Florence
(författare)
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Tombelli, SUniversity of Florence
(författare)
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Laschi, SUniversity of Florence
(författare)
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Turner, AnthonyLinköpings universitet,Biosensorer och bioelektronik,Tekniska högskolan(Swepub:liu)anttu13
(författare)
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Mascini, MUniversity of Florence
(författare)
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Marrazza, GUniversity of Florence
(författare)
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University of FlorenceBiosensorer och bioelektronik
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Electroanalysis: Wiley-VCH Verlag Berlin24:4, s. 735-7421040-03971521-4109
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