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Combined Multivaria...
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Zhao, YelinLinköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet
(författare)
Combined Multivariate and Pathway Analyses Show That Allergen-Induced Gene Expression Changes in CD4(+) T Cells Are Reversed by Glucocorticoids
- Artikel/kapitelEngelska2012
Förlag, utgivningsår, omfång ...
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2012-06-12
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Public Library of Science,2012
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electronicrdacarrier
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LIBRIS-ID:oai:DiVA.org:liu-79798
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-79798URI
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https://doi.org/10.1371/journal.pone.0039016DOI
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https://gup.ub.gu.se/publication/160363URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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Background: Glucocorticoids (GCs) play a key role in the treatment of allergy. However, the genome-wide effects of GCs on gene expression in allergen-challenged CD4(+) T cells have not been described. The aim of this study was to perform a genome-wide analysis to investigate whether allergen-induced gene expression changes in CD4(+) T cells could be reversed by GCs. Methodology/Principal Findings: Gene expression microarray analysis was performed to profile gene expression in diluent( D), allergen- (A), and allergen + hydrocortisone- (T) challenged CD4(+) T cells from patients with seasonal allergic rhinitis. Principal component analysis (PCA) showed good separation of the three groups. To identify the correlation between changes in gene expression in allergen-challenged CD4(+) T cells before and after GC treatment, we performed orthogonal partial least squares discriminant analysis (OPLS-DA) followed by Pearson correlation analysis. This revealed that allergen-induced genes were widely reversed by GC treatment (r = -0.77, Pless than0.0001). We extracted 547 genes reversed by GC treatment from OPLS-DA models based on their high contribution to the discrimination and found that those genes belonged to several different inflammatory pathways including TNFR2 Signalling, Interferon Signalling, Glucocorticoid Receptor Signalling and T Helper Cell Differentiation. The results were supported by gene expression microarray analyses of two independent materials. Conclusions/Significance: Allergen-induced gene expression changes in CD4(+) T cells were reversed by treatment with glucocorticoids. The top allergen-induced genes that reversed by GC treatment belonged to several inflammatory pathways and genes of known or potential relevance for allergy.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Wang, HuiGothenburg University,Göteborgs universitet,Linköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet,Institutionen för kliniska vetenskaper, Avdelningen för pediatrik,Institute of Clinical Sciences, Department of Pediatrics(Swepub:gu)xwanhu
(författare)
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Gustafsson, MikaLinköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet(Swepub:liu)mikgu75
(författare)
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Muraro, AntonellaUniversity of Padua, Italy
(författare)
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Bruhn, SörenLinköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet(Swepub:liu)sorbr27
(författare)
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Benson, MikaelLinköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet(Swepub:gu)xbenmi
(författare)
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Linköpings universitetInstitutionen för klinisk och experimentell medicin
(creator_code:org_t)
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Ingår i:PLOS ONE: Public Library of Science7:61932-6203
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