Sökning: onr:"swepub:oai:DiVA.org:liu-81981" > Combining gene expr...
Fältnamn | Indikatorer | Metadata |
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000 | 03605naa a2200349 4500 | |
001 | oai:DiVA.org:liu-81981 | |
003 | SwePub | |
008 | 120927s2012 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-819812 URI |
024 | 7 | a https://doi.org/10.1016/j.cyto.2012.08.0092 DOI |
040 | a (SwePub)liu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Bruhn, Sören,d 1976-u Linköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet,Center for individualized medication4 aut0 (Swepub:liu)sorbr27 |
245 | 1 0 | a Combining gene expression microarray- and cluster analysis with sequence-based predictions to identify regulators of IL-13 in allergy |
264 | 1 | b Elsevier,c 2012 |
338 | a print2 rdacarrier | |
500 | a funding agencies|European Commission|223367|Swedish Medical Research Council|| | |
520 | a The Th2 cytokine IL-13 plays a key role in allergy, by regulating IgE, airway hyper secretion, eosinophils and mast cells. In this study, we aimed to identify novel transcription factors (TFs) that potentially regulated IL-13. We analyzed Th2 polarized naïve T cells from four different blood donors with gene expression microarrays to find clusters of genes that were correlated or anti-correlated with IL13. These clusters were further filtered, by selecting genes that were functionally related. In these clusters, we identified three transcription factors (TFs) that were predicted to regulate the expression of IL13, namely CEBPB, E2F6 and AHR. siRNA mediated knockdowns of these TFs in naïve polarized T cells showed significant increases of IL13, following knockdown of CEBPB and E2F6, but not AHR. This suggested an inhibitory role of CEBPB and E2F6 in the regulation of IL13 and allergy. This was supported by analysis of E2F6, but not CEBPB, in allergen-challenged CD4+ T cells from six allergic patients and six healthy controls, which showed decreased expression of E2F6 in patients. In summary, our findings indicate an inhibitory role of E2F6 in the regulation of IL-13 and allergy. The analytical approach may be generally applicable to elucidate the complex regulatory patterns in Th2 cell polarization and allergy. | |
653 | a Allergy Interleukin-13 Transcription factor E2F6 | |
700 | 1 | a Katzenellenbogen, Marku Linköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet,Center for individualized medication4 aut |
700 | 1 | a Gustafsson, Mika,d 1977-u Linköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet,Center for individualized medication4 aut0 (Swepub:liu)mikgu75 |
700 | 1 | a Krönke, Andreau Cenix BioScience GmbH, Dresden, Germany4 aut |
700 | 1 | a Sönnichsen, Birteu Cenix BioScience GmbH, Dresden, Germany4 aut |
700 | 1 | a Zhang, Huanu Linköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet,Center for individualized medication4 aut0 (Swepub:liu)huazh47 |
700 | 1 | a Benson, Mikaelu Linköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet,Center for individualized medication4 aut0 (Swepub:liu)mikbe05 |
710 | 2 | a Linköpings universitetb Institutionen för klinisk och experimentell medicin4 org |
773 | 0 | t Cytokined : Elsevierg 60:3, s. 736-740q 60:3<736-740x 1043-4666x 1096-0023 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-81981 |
856 | 4 8 | u https://doi.org/10.1016/j.cyto.2012.08.009 |
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