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Mechanism of apopto...
Mechanism of apoptosis induced by S100A8/A9 in colon cancer cell lines : the role of ROS and the effect of metal ions
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- Ghavami, Saeid (författare)
- Tarbiat Modarres University, Tehran, Iran
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- Kerkhoff, C. (författare)
- Institute of Experimental Dermatology, Münster, Germany
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- Los, Marek Jan (författare)
- Institute of Experimental Dermatology, Münster, Germany
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- Hashemi, M. (författare)
- Tarbiat Modarres University, Tehran, Iran
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- Sorg, C. (författare)
- Institute of Experimental Dermatology, Münster, Germany
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- Karami-Tehrani, F. (författare)
- Tarbiat Modarres University, Tehran, Iran
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(creator_code:org_t)
- 2004-04-01
- 2004
- Engelska.
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Ingår i: Journal of Leukocyte Biology. - : Oxford University Press (OUP). - 0741-5400 .- 1938-3673. ; 76:1, s. 169-175
- Relaterad länk:
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https://liu.diva-por... (primary) (Raw object)
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https://jlb.onlineli...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The protein complex S100A8/A9, abundant in the cytosol of neutrophils, is secreted from the cells upon cellular activation and induces apoptosis in tumor cell lines and normal fibroblasts in a zinc-reversible manner. In the present study, we present evidence that the S100A8/A9 also exerts its apoptotic effect by a zinc-independent mechanism. Treatment of the colon carcinoma cells with different concentrations of human SI00A8/A9 or the metal ion chelator diethylenetriaminepentacetic acid (DTPA) resulted in a significant increase of cell death. Annexin V/phosphatidylinositol and Hoechst 33258 staining revealed that cell death was mainly of the apoptotic type. A significant increase in the activity of caspase-3 and -9 was observed in both cell lines after treatment. Caspase-8 activation was negligible in both cell lines. The cytotoxicity/apoptotic effect of human SI00A8/A9 and DTPA was inhibited significantly 2 2 (P<0.05) by Zn+2 and Cu+2, more effectively than by Ca2+ and Mg2+. The antioxidant N-acetyl-L-cysteine inhibited the cytotoxicity/apoptotic effect of SI00A8/A9 and DTPA. However, as a result of the different time-courses of both agents and that the S100A8/A9-induced apoptosis was not completely reversed, we conclude that S100A8/A9 exerts its apoptotic effect on two colon carcinoma cell lines through a dual mechanism: one via zinc exclusion from the target cells and the other through a yet-undefined mechanism, probably relaying on the cell-surface receptor(s).
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Antimicrobial activity
- arachidonic-acid
- calcium-binding protein
- calprotectin
- caspase activation
- cytotoxic peptides
- endothelial-cells
- human neutrophils
- inhibitory-factor
- peritoneal-exudate cells
- polymorphonuclear neutrophils
- s100 protein
- zinc-binding
- zinc-binding protein
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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