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Mechanism of apopto...
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Ghavami, SaeidTarbiat Modarres University, Tehran, Iran
(författare)
Mechanism of apoptosis induced by S100A8/A9 in colon cancer cell lines : the role of ROS and the effect of metal ions
- Artikel/kapitelEngelska2004
Förlag, utgivningsår, omfång ...
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2004-04-01
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Oxford University Press (OUP),2004
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electronicrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:liu-87002
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-87002URI
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https://doi.org/10.1189/jlb.0903435DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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The protein complex S100A8/A9, abundant in the cytosol of neutrophils, is secreted from the cells upon cellular activation and induces apoptosis in tumor cell lines and normal fibroblasts in a zinc-reversible manner. In the present study, we present evidence that the S100A8/A9 also exerts its apoptotic effect by a zinc-independent mechanism. Treatment of the colon carcinoma cells with different concentrations of human SI00A8/A9 or the metal ion chelator diethylenetriaminepentacetic acid (DTPA) resulted in a significant increase of cell death. Annexin V/phosphatidylinositol and Hoechst 33258 staining revealed that cell death was mainly of the apoptotic type. A significant increase in the activity of caspase-3 and -9 was observed in both cell lines after treatment. Caspase-8 activation was negligible in both cell lines. The cytotoxicity/apoptotic effect of human SI00A8/A9 and DTPA was inhibited significantly 2 2 (P<0.05) by Zn+2 and Cu+2, more effectively than by Ca2+ and Mg2+. The antioxidant N-acetyl-L-cysteine inhibited the cytotoxicity/apoptotic effect of SI00A8/A9 and DTPA. However, as a result of the different time-courses of both agents and that the S100A8/A9-induced apoptosis was not completely reversed, we conclude that S100A8/A9 exerts its apoptotic effect on two colon carcinoma cell lines through a dual mechanism: one via zinc exclusion from the target cells and the other through a yet-undefined mechanism, probably relaying on the cell-surface receptor(s).
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Kerkhoff, C.Institute of Experimental Dermatology, Münster, Germany
(författare)
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Los, Marek JanInstitute of Experimental Dermatology, Münster, Germany(Swepub:liu)marlo14
(författare)
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Hashemi, M.Tarbiat Modarres University, Tehran, Iran
(författare)
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Sorg, C.Institute of Experimental Dermatology, Münster, Germany
(författare)
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Karami-Tehrani, F.Tarbiat Modarres University, Tehran, Iran
(författare)
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Tarbiat Modarres University, Tehran, IranInstitute of Experimental Dermatology, Münster, Germany
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Journal of Leukocyte Biology: Oxford University Press (OUP)76:1, s. 169-1750741-54001938-3673
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