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Sökning: onr:"swepub:oai:DiVA.org:liu-95509" > Sex sorting increas...

  • Balao da Silva, C M.University of Extremadura Caceres, Spain (författare)

Sex sorting increases the permeability of the membrane of stallion spermatozoa

  • Artikel/kapitelEngelska2013

Förlag, utgivningsår, omfång ...

  • Elsevier Masson,2013
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:liu-95509
  • https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-95509URI
  • https://doi.org/10.1016/j.anireprosci.2013.02.021DOI
  • https://res.slu.se/id/publ/40723URI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • Funding Agencies|Ministerio de Ciencia e Innovacion-FEDER in Madrid, Spain|AGL 2010-20758BFU2011-30261|Junta de Extremadura, Merida, Spain-FEDER|10010PCE1002|
  • At present, the only repeatable means of selecting the sex of offspring is the Beltsville semen sorting technology using flow cytometry (FC). This technology has reached commercial status in the bovine industry and substantial advances have occurred recently in swine and ovine species. In the equine species, however, the technology is not as well developed. To better understand the changes induced in stallion spermatozoa during the sorting procedure, pooled sperm samples were sorted: sperm motility and kinematics were assessed using computer assisted sperm analysis, sperm membrane integrity was assessed using the YoPro-1 assay, while plasmalemmal stability and lipid architecture were assessed using Merocyanine 540/SYTOX green and Annexin-V, respectively. Lipid peroxidation was also investigated with the probe Bodipy(581/591)-C11. All assays were performed shortly after collection, after incubation and after sex sorting using FC. In order to characterize potential molecular mechanisms implicated in sperm damage, an apoptosis protein antibody dot plot array analysis was performed before and after sorting. While the percentage of total motile sperm remained unchanged, sex sorting reduced the percentages of progressive motile spermatozoa and of rapid spermatozoa as well as curvilinear velocity (VCL). Sperm membranes responded to sorting with an increase in the percentage of YoPro-1 positive cells, suggesting the sorted spermatozoa had a reduced energy status that was confirmed by measuring intracellular ATP content.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Ortega Ferrusola, CUniversity of Extremadura Caceres, Spain (författare)
  • Morillo Rodriguez, AUniversity of Extremadura Caceres, Spain (författare)
  • Morrell, JaneSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences,University of Extremadura Caceres, Spain,Swedish University of Agriculture Science, Sweden(Swepub:slu)50646 (författare)
  • Plaza Davila, MUniversity of Extremadura Caceres, Spain (författare)
  • Morrell, J M.Swedish University of Agriculture Science, Sweden (författare)
  • Rodriguez Martinez, HLinköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Hälsouniversitetet(Swepub:liu)herro55 (författare)
  • Tapia, J A.University of Extremadura Caceres, Spain (författare)
  • Aparicio, I M.University of Extremadura Caceres, Spain (författare)
  • Pena, F -j.University of Extremadura Caceres, Spain (författare)
  • University of Extremadura Caceres, SpainInstitutionen för kliniska vetenskaper (KV) (creator_code:org_t)
  • Sveriges lantbruksuniversitet

Sammanhörande titlar

  • Ingår i:Animal Reproduction Science: Elsevier Masson138:3-4, s. 241-2510378-43201873-2232

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