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Cloning, expression...
Cloning, expression and characterization of mouse spermatid specific thioredoxin-1 gene and protein
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- Jiménez, Alberto (författare)
- Karolinska Institutet
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- Oko, Richard (författare)
- Queen's University, Kingston, Ontario, Canada
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- Gustafsson, Jan-Åke (författare)
- Center for Biotechnology, Department of Biosciences at NOVUM, Karolinska Institutet, Huddinge, Sweden
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- Spyrou, Giannis (författare)
- Center for Biotechnology, Department of Biosciences at NOVUM, Karolinska Institutet, Huddinge, Sweden
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- Pelto-Huikko, Markku (författare)
- Tampere University Medical School and Tampere University Hospital, Finland
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- Miranda-Vizuete, Antonio (författare)
- Center for Biotechnology, Department of Biosciences at NOVUM, Karolinska Institutet, Huddinge, Sweden
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(creator_code:org_t)
- Oxford University Press, 2002
- 2002
- Engelska.
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Ingår i: Molecular human reproduction. - : Oxford University Press. - 1360-9947 .- 1460-2407. ; 8:8, s. 710-718
- Relaterad länk:
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https://academic.oup...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Thioredoxins are proteins that participate in different cellular processes via redox-mediated reactions. For humans, we have recently described two novel members of this family that display a male germ cell specific expression pattern, named spermatid specific thioredoxin (Sptrx-1 and Sptrx-2 respectively). We report here the cloning and characterization of the mouse Sptrx-1 gene and protein, which are similar to those described for the human orthologue. The mouse Sptrx-1 open reading frame encodes for a protein of 462 aa composed of an N-terminal repetitive domain of a 15 residue motif followed by a C-terminal domain typical of thioredoxins. The mouse Sptrx-1 gene sequence is interrupted by only one intron of 525 bp located in the 5'-UTR, and using fluorescence in-situ hybridization we have mapped its chromosomal location to 17E1.2-1.3. Northern blot analysis identified the testis as the only tissue expressing mouse Sptrx-1 mRNA, and by in-situ hybridization we found a strong labelling in the testicular seminiferous tubules, mostly in the round spermatids. Affinity purified antibodies against human Sptrx-1 crossreacted well with the mouse protein confirming its expression in seminiferous tubules at the later stages of spermiogenesis. Recombinant mouse Sptrx-1 displayed protein disulphide reducing activity in an enzymatic assay coupled to NADPH and thioredoxin reductase. The availability of the mouse Sptrx-1 gene sequence is the first step towards the generation of knock-out mice, whose characterization will provide significant information regarding the in-vivo function of Sptrx-1 and its possible implication in several sperm anomalies.
Nyckelord
- fibrous sheath
- sperm
- testis
- thioredoxin
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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