Sökning: onr:"swepub:oai:DiVA.org:lnu-103501" >
Interleukin 8 Elici...
Interleukin 8 Elicits Rapid Physiological Changes in Neutrophils That Are Altered by Inflammatory Conditions
-
- Bernhard, Stefan (författare)
- Univ Hosp Ulm, Inst Clin & Expt Trauma Immunol, Ulm, Germany.
-
- Hug, Stefan (författare)
- Univ Hosp Ulm, Inst Clin & Expt Trauma Immunol, Ulm, Germany.
-
- Stratmann, Alexander Elias Paul (författare)
- Univ Hosp Ulm, Inst Clin & Expt Trauma Immunol, Ulm, Germany.
-
visa fler...
-
- Erber, Maike (författare)
- Univ Hosp Ulm, Inst Clin & Expt Trauma Immunol, Ulm, Germany.
-
- Vidoni, Laura (författare)
- Univ Hosp Ulm, Inst Clin & Expt Trauma Immunol, Ulm, Germany.
-
- Knapp, Christiane Leonie (författare)
- Univ Hosp Ulm, Inst Clin & Expt Trauma Immunol, Ulm, Germany.
-
- Thomass, Bertram Dietrich (författare)
- Univ Hosp Ulm, Inst Clin & Expt Trauma Immunol, Ulm, Germany.
-
- Fauler, Michael (författare)
- Univ Ulm, Inst Gen Physiol, Ulm, Germany.
-
- Nilsson, Bo (författare)
- Uppsala universitet,Klinisk immunologi
-
- Nilsson Ekdahl, Kristina (författare)
- Uppsala universitet,Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Uppsala University, Sweden,Linnaeus Ctr Biomat Chem, BMC,Klinisk immunologi,Linnaeus Univ, Ctr Biomat Chem, Kalmar, Sweden.
-
- Foehr, Karl (författare)
- Univ Hosp Ulm, Dept Anesthesiol & Intens Care Med, Ulm, Germany.
-
- Braun, Christian Karl (författare)
- Univ Hosp Ulm, Inst Clin & Expt Trauma Immunol, Ulm, Germany.;Univ Hosp Ulm, Dept Pediat & Adolescent Med, Ulm, Germany.
-
- Wohlgemuth, Lisa (författare)
- Univ Hosp Ulm, Inst Clin & Expt Trauma Immunol, Ulm, Germany.
-
- Huber-Lang, Markus (författare)
- Univ Hosp Ulm, Inst Clin & Expt Trauma Immunol, Ulm, Germany.
-
- Messerer, David Alexander Christian (författare)
- Univ Hosp Ulm, Inst Clin & Expt Trauma Immunol, Ulm, Germany.;Univ Hosp Ulm, Dept Anesthesiol & Intens Care Med, Ulm, Germany.
-
visa färre...
-
Univ Hosp Ulm, Inst Clin & Expt Trauma Immunol, Ulm, Germany Univ Ulm, Inst Gen Physiol, Ulm, Germany. (creator_code:org_t)
- 2021-04-15
- 2021
- Engelska.
-
Ingår i: Journal of Innate Immunity. - : S. Karger. - 1662-811X .- 1662-8128. ; 13, s. 225-241
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://www.karger.c...
-
https://uu.diva-port... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
visa färre...
Abstract
Ämnesord
Stäng
- A sufficient response of neutrophil granulocytes stimulated by interleukin (IL)-8 is vital during systemic inflammation, for example, in sepsis or severe trauma. Moreover, IL-8 is clinically used as biomarker of inflammatory processes. However, the effects of IL-8 on cellular key regulators of neutrophil properties such as the intracellular pH (pH(i)) in dependence of ion transport proteins and during inflammation remain to be elucidated. Therefore, we investigated in detail the fundamental changes in pH(i), cellular shape, and chemotactic activity elicited by IL-8. Using flow cytometric methods, we determined that the IL-8-induced cellular activity was largely dependent on specific ion channels and transporters, such as the sodium-proton exchanger 1 (NHE1) and non-NHE1-dependent sodium flux. Exposing neutrophils in vitro to a proinflammatory micromilieu with N-formyl-Met-Leu-Phe, LPS, or IL-8 resulted in a diminished response regarding the increase in cellular size and pH. The detailed kinetics of the reduced reactivity of the neutrophil granulocytes could be illustrated in a near-real-time flow cytometric measurement. Last, the LPS-mediated impairment of the IL-8-induced response in neutrophils was confirmed in a translational, animal-free human whole blood model. Overall, we provide novel mechanistic insights for the interaction of IL-8 with neutrophil granulocytes and report in detail about its alteration during systemic inflammation.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- Neutrophil granulocytes
- Intracellular pH
- Lipopolysaccharide
- Sodium-proton exchanger 1
- Flow cytometry
- Interleukin 8
- Immunologi
- Immunology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Bernhard, Stefan
-
Hug, Stefan
-
Stratmann, Alexa ...
-
Erber, Maike
-
Vidoni, Laura
-
Knapp, Christian ...
-
visa fler...
-
Thomass, Bertram ...
-
Fauler, Michael
-
Nilsson, Bo
-
Nilsson Ekdahl, ...
-
Foehr, Karl
-
Braun, Christian ...
-
Wohlgemuth, Lisa
-
Huber-Lang, Mark ...
-
Messerer, David ...
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinska och f ...
-
och Immunologi inom ...
- Artiklar i publikationen
-
Journal of Innat ...
- Av lärosätet
-
Linnéuniversitetet
-
Uppsala universitet